Recombinant Human MYOC protein, His-tagged

• Cat.No.: MYOC-6991H
• Product Overview: Recombinant Human MYOC (Arg33-Met504) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Myocilin, trabecular meshwork inducible glucocorticoid response is also known as MYOC, GLC1A, GPOA, JOAG, JOAG1, TIGR. Myocilin/MYOC contains one olfactomedin-like domain. Myocilin is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma.
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Arg33
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 32 kDa. The protein migrates as 33 kDa under reducing (R) condition (SDS-PAGE) due to most of the MYOC was cleaved at Glu 214, generating a C-terminal fragment.
• Protein length: Arg33-Met504
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: MYOC
• Official Symbol: MYOC
• Synonyms: MYOC; myocilin, trabecular meshwork inducible glucocorticoid response; GLC1A; myocilin; JOAG1; TIGR; mutated trabecular meshwork-induced glucocorticoid response protein; GPOA; JOAG
• Gene ID: 4653
• mRNA Refseq: NM_000261
• Protein Refseq: NP_000252
• MIM: 601652
• UniProt ID: Q99972