Active Recombinant Human Peptidylprolyl Isomerase (cyclophilin)-Like 3, His-tagged

• Cat.No.: PPIL3-2491H
• Product Overview: Recombinant human PPIL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 20.3 kDa.

Specification

• Description: PPIL3, also known as peptidyl-prolyl cis-trans isomerase-like 3, is a member of the cyclophilin family that catalyzes the cis-trans isomerization of peptidylprolyl imide bonds in oligopeptides. It has been proposed to act either as catalysts or as molecular chaperones in protein-folding events.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid 20mM Tris-HCI buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT.
• Bio-activity: Specific activity is >280 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
• Molecular Mass: 20.3 kDa (181aa) confirmed by MALDI-TOF.
• Protein length: 1-161aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MSVTLHTDVG DIKIEVFCER TPKTCENFLA LCASNYYNGC IFHRNIKGFM VQTGDPTGTG RGGNSIWGKK FEDEYSEYLK HNVRGVVSMA NNGPNTNGSQ FFITYGKQPH LDMKYTVFGK VIDGLETLDE LEKLPVNEKT YRPLNDVHIK DITIHANPFA Q
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PPIL3 peptidylprolyl isomerase (cyclophilin)-like 3 [ Homo sapiens ]
• Official Symbol: PPIL3
• Synonyms: PPIL3; peptidylprolyl isomerase (cyclophilin)-like 3; peptidyl-prolyl cis-trans isomerase-like 3; Cyclophilin J; CyPJ; PPIase; cyclophilin J; rotamase PPIL3; PPIase-like protein 3; cyclophilin-like protein 3; cyclophilin-like protein PPIL3; peptidylprolyl cis-trans isomerase-like protein 3; CYPJ
• Gene ID: 53938
• mRNA Refseq: NM_032472
• Protein Refseq: NP_115861
• UniProt ID: Q9H2H8
• Chromosome Location: 2q33.1
• Function: isomerase activity; peptidyl-prolyl cis-trans isomerase activity; protein binding