Recombinant Human Linker For Activation Of T Cells, His-tagged

• Cat.No.: LAT-6882H
• Product Overview: Recombinant human LAT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 24.4 kDa.

Specification

• Description: LAT is an integral membrane protein which is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 1mM DTT.
• Molecular Mass: 24.4 kDa (227aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher).
• Protein length: 28-233aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MHCHRLPGSY DSTSSDSLYP RGIQFKRPHT VAPWPPAYPP VTSYPPLSQP DLLPIPRSPQ PLGGSHRTPS SRRDSDGANS VASYENEEPA CEDADEDEDD YHNPGYLVVL PDSTPATSTA APSAPALSTP GIRDSAFSME SIDDYVNVPE SGESAEASLD GSREYVNVSQ ELHPGAAKTE PAALSSQEAE EVEEEGAPDY ENLQELN
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford)

Gene Information

• Gene Name: LAT linker for activation of T cells [ Homo sapiens ]
• Official Symbol: LAT
• Synonyms: LAT; linker for activation of T cells; linker for activation of T-cells family member 1; LAT1; transmembrane adaptor; p36-38; 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; linker for activation of T cells, transmembrane adaptor; pp36
• Gene ID: 27040
• mRNA Refseq: NM_001014987
• Protein Refseq: NP_001014987
• MIM: 602354
• UniProt ID: O43561
• Chromosome Location: 16q13
• Pathway: Adaptive Immune System, organism-specific biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem; Fc epsilon RI signaling pathway, conserved biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Fc-epsilon receptor I signaling in mast cells, organism-specific biosystem; GPVI-mediated activation cascade, organism-specific biosystem
• Function: SH3/SH2 adaptor activity; protein binding; protein kinase binding