Recombinant Human DnaJ (Hsp40) Homolog, Subfamily B, Member 8, His-tagged

• Cat.No.: DNAJB8-6898H
• Product Overview: Recombinant human DNAJB8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 27.8 kDa.

Specification

• Description: DNAJB8, also known as dnaJ homolog subfamily B member 8, is superior suppressors of aggregation and toxicity of disease-associated polyglutamine proteins. DNAJB8 is (de)acetylated at two conserved C-terminal lysines that are not involved in substrate binding, but do play a role in suppressing protein aggregation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT.
• Molecular Mass: 27.8 kDa (252aa), confirmed by MALDI-TOF
• Protein length: 1-232aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MANYYEVLGV QA SASPEDIK KAYRKLALRW HPDKNPDNKE EAEKKFKLVS EAYEVLSDSK KRSLYDRAGC DSWRAGGGAS TPYHSPFDTG YTFRNPEDIF REFFGGLDPF SFEFWDSPFN SDRGGRGHGL RG AFSAGFGE FPAFMEAFSS FNMLGCSGGS HTTFSSTSFG GSSSGSSGFK SVMSSTEMIN GH KVTTKRIV ENGQERVEVE EDGQLKSVTV NGKEQLKWMD SK
• Purity: >90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4℃ short term (1-2 weeks). For long term storage, aliquot and store at -20℃ or -70℃. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: DNAJB8 DnaJ (Hsp40) homolog, subfamily B, member 8 [ Homo sapiens ]
• Official Symbol: DNAJB8
• Synonyms: DNAJB8; DnaJ (Hsp40) homolog, subfamily B, member 8; dnaJ homolog subfamily B member 8; MGC33884; DnaJ homolog, subfamily B, member 8; DJ6
• Gene ID: 165721
• mRNA Refseq: NM_153330
• Protein Refseq: NP_699161
• MIM: 611337
• UniProt ID: Q8NHS0
• Chromosome Location: 3q21.3
• Function: heat shock protein binding; unfolded protein binding