Recombinant Human Glutamate-Cysteine Ligase, Modifier Subunit, His-tagged
Cat.No. : | GCLM-2522H |
Product Overview : | Recombinant human GCLM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
- Specification
- Gene Information
- Related Products
Description : | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.15M NaCl. |
Molecular Mass : | 33.3kDa (298aa), confirmed by MALDI-TOF |
Protein length : | 1-274aa |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSHMGTDSR AAKALLARAR TLHLQTGNLL NWGRLRKKCP STHSEELHDC IQKTLNEWSS QINPDLVREF PDVLECTVSH AVEKINPDER EEMKVSAKLF IVESNSSSST RSAVDMACSV LGVAQLDSVI IASPPIEDGV NLSLEHLQPY WEELENLVQS KKIVAIGTSD LDKTQLEQLY QWAQVKPNSN QVNLASCCVM PPDLTAFAKQ FDIQLLTHND PKELLSEASF QEALQESIPD IQAHEWVPLW LLRYSVIVKS RGIIKSKGYI LQAKRRGS |
Purity : | >90% by SDS - PAGE |
Applications : | SDS-PAGE |
Storage : | Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles. |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Gene Name : | GCLM glutamate-cysteine ligase, modifier subunit [ Homo sapiens ] |
Official Symbol : | GCLM |
Synonyms : | GCLM; glutamate-cysteine ligase, modifier subunit; GLCLR; glutamate--cysteine ligase regulatory subunit; gamma glutamylcysteine synthetase; GCS light chain; GSC light chain; gamma-ECS regulatory subunit; glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; gamma-glutamylcysteine synthetase regulatory subunit; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD); |
Gene ID : | 2730 |
mRNA Refseq : | NM_002061 |
Protein Refseq : | NP_002052 |
MIM : | 601176 |
UniProt ID : | P48507 |
Chromosome Location : | 1p21 |
Pathway : | Biological oxidations, organism-specific biosystem; Glutathione biosynthesis, glutamate => glutathione, organism-specific biosystem; Glutathione biosynthesis, glutamate => glutathione, conserved biosystem; Glutathione conjugation, organism-specific biosystem; Glutathione metabolism, organism-specific biosystem; |
Function : | contributes_to glutamate-cysteine ligase activity; contributes_to glutamate-cysteine ligase activity; glutamate-cysteine ligase catalytic subunit binding; protein heterodimerization activity; |
Products Types
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◆ Lysates | ||
GCLM-5982HCL | Recombinant Human GCLM 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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