Recombinant Human Ephrin-B3, His-tagged

• Cat.No.: EFNB3-6965H
• Product Overview: Recombinant human EFNB3 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Specification

• Description: EFNB3, also known as ephrin-B3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. It binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to, and induce the collapse of, commissural axons/growth cones in vitro.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 2M urea.
• Molecular Mass: 24.6 kDa (224aa)
• Protein length: 28-226aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMLSLEP VYWNSANKRF QAEGGYVLYP QIGDRLDLLC PRARPPGPHS SPNYEFYKLY LVGGAQGRRC EAPPAPNLLL TCDRPDLDLR FTIKFQEYSP NLWGHEFRSH HDYYIIATSD GTREGLESLQ GGVCLTRGMK VLLRVGQSPR GGAVPRKPVS EMPMERDRGA AHSLEPGKEN LPGDPTSNAT SRGAEGPLPP PSMP
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: EFNB3 ephrin-B3 [ Homo sapiens ]
• Official Symbol: EFNB3
• Synonyms: EFNB3; ephrin-B3; EPLG8; LERK 8; Ephrin B3; eph-related receptor tyrosine kinase ligand 8; EPH-related receptor transmembrane ligand ELK-L3; EFL6; LERK8
• Gene ID: 1949
• mRNA Refseq: NM_001406
• Protein Refseq: NP_001397
• MIM: 602297
• UniProt ID: Q15768
• Chromosome Location: 17p13.1
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; EPHB forward signaling, organism-specific biosystem
• Function: ephrin receptor binding; transmembrane-ephrin receptor activity