Recombinant Human Guanine Nucleotide Binding Protein (G Protein), Alpha Inhibiting Activity Polypeptide 2, His-tagged

• Cat.No.: GNAI2-7152H
• Product Overview: Recombinant human GNAI2 proetin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 42 kDa.

Specification

• Description: GNAI2 is an alpha subunit of guanine nucleotide binding proteins (G proteins). The protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only two are known so far.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol.
• Molecular Mass: 42 kDa (375aa) confirmed by MALDI-TOF
• Protein length: 1-355aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPSR ADDARQLFAL SCTAEEQGVL PDDLSGVIRR LWADHGVQAC FGRSREYQLN DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL FDSICNNKWF TDTSIILFLN KKDLFEEKIT HSPLTICFPE YTGANKYDEA ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK DCGLF
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GNAI2 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 [ Homo sapiens ]
• Official Symbol: GNAI2
• Synonyms: GNAI2; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2; GNAI2B; guanine nucleotide-binding protein G(i) subunit alpha-2; GIP; GTP binding regulatory protein Gi alpha 2 chain; adenylate cyclase-inhibiting G alpha protein; GTP-binding regulatory protein Gi alpha-2 chain; guanine nucleotide-binding protein G(i), alpha-2 subunit; H_LUCA15.1; H_LUCA16.1
• Gene ID: 2771
• mRNA Refseq: NM_001166425
• Protein Refseq: NP_001159897
• MIM: 139360
• UniProt ID: P04899
• Chromosome Location: 3p21.31
• Pathway: ADP signalling through P2Y purinoceptor 12, organism-specific biosystem; Activation of GABAB receptors, organism-specific biosystem; Adenylate cyclase inhibitory pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; CXCR3-mediated signaling events, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem
• Function: G-protein beta/gamma-subunit complex binding; G-protein coupled receptor binding; GTP binding; GTPase activity; GTPase activity; guanyl nucleotide binding; guanyl-nucleotide exchange factor activity; metal ion binding; nucleotide binding; phosphatidylinositol phospholipase C activity; protein binding; signal transducer activity