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Recombinant Human Neutrophil Cytosolic Factor 4, 40kDa, His-tagged

Cat.No. : NCF4-7201H
Product Overview : Recombinant human NCF4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 41.1 kDa.
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Description : NCF4 is a cytosolic regulatory of the superoxide-producing phagocyte NADPH-oxidase, amulticomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integratedcatalytic core of the enzyme system.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
Molecular Mass : 41.1 kDa (359aa) confirmed by MALDI-TOF
Protein length : 1-339aa
AA Sequence : MGSSHHHHHH SSGLVPRGSHMAVAQQLRAE SDFEQLPDDV AISANIADIE EKRGFTSHFV FVIEVKTKGG SKYLIYRRYR QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE IAEMRIPALN AYMKSLLSLP VWVLMDEDVR IFFYQSPYDS EQVPQALRRL RPRTRKVKSV SPQGNSVDRM AAPRAEALFD FTGNSKLELN FKAGDVIFLL SRINKDWLEG TVRGATGIFP LSFVKILKDF PEEDDPTNWL RCYYYEDTIS TIKDIAVEED LSSTPLLKDL LELTRREFQR EDIALNYRDA EGDLVRLLSD EDVALMVRQA RGLPSQKRLF PWKLHITQKD NYRVYNTMP
Purity : >90% by SDS - PAGE
Applications : SDS-PAGE
Storage : Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Publication :
Cytosolic PCNA interacts with p47phox and controls NADPH oxidase NOX2 activation in neutrophils (2019)
Gene Name : NCF4 neutrophil cytosolic factor 4, 40kDa [ Homo sapiens ]
Official Symbol : NCF4
Synonyms : NCF4; neutrophil cytosolic factor 4, 40kDa; neutrophil cytosolic factor 4 (40kD); neutrophil cytosol factor 4; neutrophil NADPH oxidase factor 4; p40phox; SH3PXD4; NCF-4; p40-phox; SH3 and PX domain-containing protein 4; NCF; P40PHOX; MGC3810;
Gene ID : 4689
mRNA Refseq : NM_000631
Protein Refseq : NP_000622
MIM : 601488
UniProt ID : Q15080
Chromosome Location : 22q13.1
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Cross-presentation of particulate exogenous antigens (phagosomes), organism-specific biosystem; Immune System, organism-specific biosystem; Leishmaniasis, organism-specific biosystem;
Function : phosphatidylinositol binding; protein binding; protein dimerization activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How does NCF4 protein interact with other components of the NADPH oxidase complex? 12/02/2022

NCF4 protein interacts with other subunits of the NADPH oxidase complex, such as p22phox, p47phox, and p67phox, to assemble the active enzyme complex for ROS production.

What are the implications of NCF4 protein dysfunction in the context of cancer? 05/14/2022

Dysregulation of NCF4 protein function may impact the immune response to cancer cells and potentially influence the tumor microenvironment, with potential implications for cancer progression and immunotherapy.

What are the potential therapeutic implications of targeting NCF4 protein? 06/11/2020

Targeting NCF4 protein may offer new therapeutic strategies for the treatment of immune disorders and inflammatory conditions by modulating ROS production and immune cell function.

How can NCF4 protein be measured in clinical settings? 04/22/2020

NCF4 protein levels can be measured using techniques such as western blotting, ELISA, or flow cytometry in clinical research and diagnostic laboratories.

Can variations in the NCF4 gene affect individual susceptibility to infections? 01/12/2016

Genetic variations in the NCF4 gene may influence an individual's susceptibility to infections by impacting the efficiency of phagocyte-mediated killing of pathogens.

Customer Reviews (3)

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01/27/2020

    Researchers can confidently incorporate NCF4 protein into their experiments, knowing that they have access to a reliable resource that will meet their experimental needs effectively.

    07/11/2018

      the NCF4 protein is an excellent choice for researchers due to its high quality, reliability, and the comprehensive technical support provided by the manufacturer.

      05/16/2018

        The manufacturer of NCF4 protein provides excellent technical support, ensuring that researchers receive prompt assistance and guidance when encountering any challenges or difficulties.

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