Recombinant Human Neutrophil Cytosolic Factor 4, 40kDa, His-tagged
Cat.No. : | NCF4-7201H |
Product Overview : | Recombinant human NCF4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 41.1 kDa. |
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Description : | NCF4 is a cytosolic regulatory of the superoxide-producing phagocyte NADPH-oxidase, amulticomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integratedcatalytic core of the enzyme system. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT |
Molecular Mass : | 41.1 kDa (359aa) confirmed by MALDI-TOF |
Protein length : | 1-339aa |
AA Sequence : | MGSSHHHHHH SSGLVPRGSHMAVAQQLRAE SDFEQLPDDV AISANIADIE EKRGFTSHFV FVIEVKTKGG SKYLIYRRYR QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE IAEMRIPALN AYMKSLLSLP VWVLMDEDVR IFFYQSPYDS EQVPQALRRL RPRTRKVKSV SPQGNSVDRM AAPRAEALFD FTGNSKLELN FKAGDVIFLL SRINKDWLEG TVRGATGIFP LSFVKILKDF PEEDDPTNWL RCYYYEDTIS TIKDIAVEED LSSTPLLKDL LELTRREFQR EDIALNYRDA EGDLVRLLSD EDVALMVRQA RGLPSQKRLF PWKLHITQKD NYRVYNTMP |
Purity : | >90% by SDS - PAGE |
Applications : | SDS-PAGE |
Storage : | Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Publication : |
Cytosolic PCNA interacts with p47phox and controls NADPH oxidase NOX2 activation in neutrophils (2019)
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Gene Name : | NCF4 neutrophil cytosolic factor 4, 40kDa [ Homo sapiens ] |
Official Symbol : | NCF4 |
Synonyms : | NCF4; neutrophil cytosolic factor 4, 40kDa; neutrophil cytosolic factor 4 (40kD); neutrophil cytosol factor 4; neutrophil NADPH oxidase factor 4; p40phox; SH3PXD4; NCF-4; p40-phox; SH3 and PX domain-containing protein 4; NCF; P40PHOX; MGC3810; |
Gene ID : | 4689 |
mRNA Refseq : | NM_000631 |
Protein Refseq : | NP_000622 |
MIM : | 601488 |
UniProt ID : | Q15080 |
Chromosome Location : | 22q13.1 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Cross-presentation of particulate exogenous antigens (phagosomes), organism-specific biosystem; Immune System, organism-specific biosystem; Leishmaniasis, organism-specific biosystem; |
Function : | phosphatidylinositol binding; protein binding; protein dimerization activity; |
Products Types
◆ Recombinant Protein | ||
NCF4-5939M | Recombinant Mouse NCF4 Protein, His (Fc)-Avi-tagged | +Inquiry |
NCF4-3592H | Recombinant Human NCF4 Protein, His (Fc)-Avi-tagged | +Inquiry |
NCF4-180H | Recombinant Human NCF4 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
Ncf4-4310M | Recombinant Mouse Ncf4 Protein, Myc/DDK-tagged | +Inquiry |
NCF4-10469M | Recombinant Mouse NCF4 Protein | +Inquiry |
◆ Lysates | ||
NCF4-3949HCL | Recombinant Human NCF4 293 Cell Lysate | +Inquiry |
NCF4-3948HCL | Recombinant Human NCF4 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionNCF4 protein interacts with other subunits of the NADPH oxidase complex, such as p22phox, p47phox, and p67phox, to assemble the active enzyme complex for ROS production.
Dysregulation of NCF4 protein function may impact the immune response to cancer cells and potentially influence the tumor microenvironment, with potential implications for cancer progression and immunotherapy.
Targeting NCF4 protein may offer new therapeutic strategies for the treatment of immune disorders and inflammatory conditions by modulating ROS production and immune cell function.
NCF4 protein levels can be measured using techniques such as western blotting, ELISA, or flow cytometry in clinical research and diagnostic laboratories.
Genetic variations in the NCF4 gene may influence an individual's susceptibility to infections by impacting the efficiency of phagocyte-mediated killing of pathogens.
Customer Reviews (3)
Write a reviewResearchers can confidently incorporate NCF4 protein into their experiments, knowing that they have access to a reliable resource that will meet their experimental needs effectively.
the NCF4 protein is an excellent choice for researchers due to its high quality, reliability, and the comprehensive technical support provided by the manufacturer.
The manufacturer of NCF4 protein provides excellent technical support, ensuring that researchers receive prompt assistance and guidance when encountering any challenges or difficulties.
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