Recombinant Human Proteasome (prosome, macropain) 26S Subunit, Non-ATPase, 10

• Cat.No.: PSMD10-6927H
• Product Overview: Recombinant human PSMD10 was expressed in E. coli and purified by conventional chromatography techniques. MW: 24.4 kDa.

Specification

• Description: PSMD10 is an oncoprotein commonly overexpressed in most hepatocellular carcinomas. PSMD10 interacts with S6 ATPase of the 19S regulatory particle of the 26S proteasome. It is known to play a role in the regulation of the phosphorylation of the retinoblastoma protein by CDK4, and to enhance the ubiquitinylation of p53 by MDM2. PSMD10 consists of 7 ankyrin repeats and is structurally similar to I kappa Bs. Recombinant human PSMD10 was expressed in E. coli and purified by conventional chromatography techniques.
• Source: E. coli
• Species: Human
• Tag: N/A
• Form: Liquid. In Phosphate buffered saline (pH7.4), 10% glycerol
• Molecular Mass: 24.4 kDa (226aa), confirmed by MALDI-TOF
• Protein length: 1-226aa
• AA Sequence: MEGCVSNLMV CNLAYSGKLE ELKESILADK SLATRTDQDS RTALHWACSA GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLGKGAQVN AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYEA TAMHRAAAKG NLKMIHILLY YKASTNIQDT EGNTPLHLAC DEERVEEAKL LVSQGASIYI ENKEEKTPLQ VAKGGLGLIL KRMVEG
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PSMD10 proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 [ Homo sapiens ]
• Official Symbol: PSMD10
• Synonyms: PSMD10; proteasome (prosome, macropain) 26S subunit, non-ATPase, 10; 26S proteasome non-ATPase regulatory subunit 10; gankyrin; p28; ankyrin repeat protein; 26S proteasome regulatory subunit p28; hepatocellular carcinoma-associated protein p28-II; p28(GANK); dJ889N15.2
• Gene ID: 5716
• mRNA Refseq: NM_002814
• Protein Refseq: NP_002805
• MIM: 603480
• UniProt ID: O75832
• Chromosome Location: Xq22.3
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem
• Function: protein binding; transcription factor binding