Cat.No.: |
IL18-742H |
Product Overview: |
Recombinant human IL18 was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. MW: 18.3 kDa. |
Description: |
IL18 is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. |
Source: |
E. coli |
Species: |
Human |
Tag: |
N/A |
Form: |
Liquid. In Phosphate buffer saline (pH 7.4). |
Molecular Mass: |
18.3 kDa (158aa), confirmed by MALDI-TOF. |
AA Sequence: |
MYFGKLESKL SVIRNLNDQV LFIDQGNRPL FEDMTDSDCR DNAPRTIFII SMYKDSQPRG MAVTISVKCE KISTLSCENK IISFKEMNPP DNIKDTKSDI IFFQRSVPGH DNKMQFESSS YEGYFLACEK ERDLFKLILK KEDELGDRSI MFTVQNED |
Endotoxin: |
< 1.0 eu per 1 ug of protein (determined by lal |
Purity: |
>95% by SDS - PAGE |
Applications: |
SDS-PAGE |
Storage: |
Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Concentration: |
1 mg/ml (determined by Bradford assay) |