Recombinant Human MIS12, His-tagged

• Cat.No.: MIS12-7228H

Specification

• product overview: Recombinant human MIS12 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• description: Protein MIS12 homolog, also known as MIS12, is a component of the MIS12 complex, which is required for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex consists of MIS12, DSN1, NSL1 and PMF-1. MIS12 is part of a network of complexes that provide microtubule attachment and generates pulling forces from depolymerization.
• source: E. coli
• species: Human
• tag: His
• form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2M Urea, 10% glycerol
• molecular mass: 26.5kDa (228aa)
• protein length: 1-205aa
• aa sequence: MGSSHHHHHH SSGLVPRGSH MGSMSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS
• purity: >90% by SDS - PAGE
• applications: SDS-PAGE
• storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• concentration: 1 mg/ml

Gene Information

• gene name: MIS12 MIS12, MIND kinetochore complex component, homolog (S. pombe) [ Homo sapiens ]
• official symbol: MIS12
• synonyms: MIS12; MIS12, MIND kinetochore complex component, homolog (S. pombe); MIS12, MIND kinetochore complex component, homolog (yeast); protein MIS12 homolog; hMIS12; KNTC2AP; MGC2488; MTW1; MIS12 homolog; homolog of yeast Mis12; hMis12; 2510025F08Rik
• Gene ID: 79003
• mrna refseq: NM_001258217
• protein refseq: NP_001245146
• mim: 609178
• uniprot id: Q9H081
• chromosome location: 17p13.3
• pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; DNA Replication, organism-specific biosystem; M Phase, organism-specific biosystem; Mitotic M-M/G1 phases, organism-specific biosystem; Mitotic Prometaphase, organism-specific biosystem
• function: protein binding