||Recombinant human PCNP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||PCNP (PEST proteolytic signal containing nuclear protein) is a nuclear protein implied to play a role in cell cycle regulation and tumorigenesis. This protein is ubiquitinated post-translationally by NIRF, an ubiquitin ligase. PCNP exsists as three isoforms produced by alternative splicing events and may be involved in cell cycle regulation.
||Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT
||21.5 kDa (202aa), confirmed by MALDI-TOF
||>95 % by SDS -PAGE
||1 mg/ml (determined by BCA)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMADGKA GDEKPEKSQR AGAAGGPEEE AEKPVKTKTV SSSNGGESSS RSAEKRSAEE EAADLPTKPT KISKFGFAIG SQTTKKASAI SIKLGSSKPK ETVPTLAPKT LSVAAAFNED EDSEPEEMPP EAKMRMKNIG RDTPTSAGPN SFNKGKHGFS DNQKLWERNI KSHLGNVHDQ DN
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.