Active Recombinant Rat Egfr, His-tagged

• Cat.No.: Egfr-7433R
• Product Overview: A DNA sequence encoding the rat EGFR(Met1-Ser646) was expressed in Human Cells, with a His tag at the C-terminus.

Specification

• Description: As a member of the epidermal growth factor receptor tyrosine kinases subfamily, EGF R is a type Ⅰ transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR signaling plays a crucial role in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR homo- or hetero- dimerization, initiates subsequent tyrosine autophosphorylation and various downstream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling has been shown to exert actions on carcinogenesis and disease progression, and thus EGFR was proposed as a target for cancer therapy currently.
• Source: Human Cells
• Species: Rat
• Tag: His
• Predicted N Terminal: Leu 25
• Form: Lyophilized from a 0.2μM filtered solution of PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind recombinant Human EGF-Fc in a functional ELISA. 2. Measured by its ability to bind recombinant Mouse EGF-Fc in a functional ELISA.
• Molecular Mass: The recombinant rat EGFR comprises 633 amino acids and predicts a molecular mass of 70.7 kDa. The apparent molecular mass of the recombinant protein is approximately 93-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: 1.0 eu/μg of the protein as determined by the lal
• Purity: >98 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Egfr epidermal growth factor receptor [ Rattus norvegicus ]
• Official Symbol: Egfr
• Synonyms: EGFR; epidermal growth factor receptor; EGFR-related peptide; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); Errp; ERBB1; ErbB-1
• Gene ID: 24329
• mRNA Refseq: NM_031507
• Protein Refseq: NP_113695
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem
• Function: actin filament binding; enzyme binding; epidermal growth factor binding; epidermal growth factor-activated receptor activity; epidermal growth factor-activated receptor activity; epidermal growth factor-activated receptor activity; epidermal growth factor-activated receptor activity; glycoprotein binding; identical protein binding; integrin binding; kinase activity; contributes_to nitric-oxide synthase regulator activity; protein binding; protein heterodimerization activity; protein heterodimerization activity; protein kinase binding; protein phosphatase binding; protein tyrosine kinase activity; receptor binding; signal transducer activity; transmembrane signaling receptor activity