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| Cat.No.: | CSC-P0275 |
| Cell Line Description: | CHO-Anti-Human TGF beta 2 MAb stable cell line is clonally-derived from a CHO cell line, which has been transfected with an anti-human TGF beta 2 MAb gene to allow expression of the MAb. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system. |
| Background: | TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. |
| Growth Properties: | Suspension |
| Morphology: | Epithelial-like |
| Host Cell: | CHO |
| Propagation: | Complete growth medium: Serum-free MediumAtmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37 °C |
| Starting Cells From Frozen Cell Stock: | 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37 °C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. Clean the outside of the vial with 70% ethanol before opening.3. Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media. 4. Centrifuge for 3 minutes 1000 xg to pellet cells and remove the supernatant.5. Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.6. Place the cells in the 37 °C incubator with 5% CO2.7. Allow incubation for 3-4 days to reach confluence. The cells will re-attach to the surface over a period of several days in culture at 37 °C. |
| Subculturing: | 1. Centrifuge for 5 minutes 300 xg and discard culture medium.2. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.3. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37 °C.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week |
| Mycoplasma: | Mycoplasma Status: Negative (MycoAlert Kit) |
| Freeze Medium: | Complete growth medium 95%; DMSO, 5% |
| Storage: | Liquid nitrogen vapor phase |
| Preservation: | 1. Detach cells from culture dish according to the Sub-Culture Procedure.2. Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.3. Aliquot 1 mL cells into cryogenic vials.4. Place vials in a freezing container and store at –80 °C overnight.5. Transfer vials to liquid nitrogen for long term storage. If properly stored, cells should remain stable for years. |
| Safety Considerations: | The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the stable line.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship: | Dry ice |
| Product Type: | Human IgG4 |
| Product Purity: | HPLC >98%, One strip for SDS-PAGE. |
| Product Activity/Specificity: | Tested positive against native human antigen. |
| Product Storage: | It should be stored at –20 °C. Reconstituted antibody aliquots should avoid repeated freeze-thaw cycles. |
| Involvement in Disease: | Monoclonal antibody binds to TGF beta 2, for reduce scarring after glaucoma drainage surgery. |
| Gene Name: | TGFB2 transforming growth factor, beta 2 [ Homo sapiens ] |
| Official Symbol: | TGFB2 |
| Synonyms: | TGFB2; transforming growth factor, beta 2; TGF-beta2; transforming growth factor beta-2; G-TSF; cetermin; polyergin; BSC-1 cell growth inhibitor; glioblastoma-derived T-cell suppressor factor; TGF-beta-2 |
| Gene ID: | 7042 |
| mRNA Refseq: | NM_003238 |
| Protein Refseq: | NP_003229 |
| MIM: | 190220 |
| UniProt ID: | P61812 |
| Chromosome Location: | 1q41 |
| Pathway: | ATF-2 transcription factor network; Amoebiasis; Amoebiasis; Cell cycle; Cell cycle; Chagas disease (American trypanosomiasis); Chagas disease (American trypanosomiasis); |
| Function: | beta-amyloid binding; cytokine activity; growth factor activity; protein binding; contributes_to protein binding; protein heterodimerization activity; protein homodimerization activity; receptor binding; receptor signaling protein serine/threonine kinase activity; transforming growth factor beta receptor binding |
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