"O3FAR1" Related Products


Human O3FAR1-FLAG Stable Cell Line-CHO

Cat.No.: CSC-RG0245
Cell Line Description: CHO-HuO3FAR1-FLAG is clonally-derived from a CHO-K1 cell line which has been transfected with a human omega-3 fatty acid receptor 1 (O3FAR1) tagged in the N-terminus with FLAG to allow stably express of the human O3FAR1 tagged in the N-terminus with FLAG protein. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Background: GPR120 is a G-protein coupled receptor and has been shown to stimulate secretion of gut hormones GLP-1 and cholecystokinin upon binding of long chain free fatty acids. Stimulation of GPR120 by FFAs results in elevation of Ca2+ and activation of the ERK cascade which suggests interaction with the Gq family of G proteins. GPR120 is expressed in adipose tissue, proinflammatory macrophages and Kupffer cells. Stimulation of GPR120 with agonist causes broad anti-inflammatory effects in macrophages such as insulin sensitizing, antidiabetic effects and macrophage-induced tissue inflammation repression. Recent studies of the -3 FA treatment show inhibited inflammation and enhanced systemic insulin sensitivity in WT mice, but no effect in GPR120 knockout mice. This raises the possibility that targeting this receptor could have therapeutic potential in many inflammatory diseases including obesity and type 2 diabetes.
Growth Properties: Adherent
Morphology: Epithelial-like
Host Cell: CHO-K1
Cell Line Validation: 1. Gene expression: RT-PCR experiments determined specific expression of human GPR120.2. Protein expression: GPR120 expression in this cell line has been validated by WB.3. Functional validation: Functional assays.
Sub-type: GPR
Propagation: Complete growth medium: DMEM, 10% FBS, 1 μg/mL puromycinAtmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Starting Cells From Frozen Cell Stock: 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37°C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. Clean the outside of the vial with 70% ethanol before opening.3. Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media. 4. Centrifuge for 3 minutes 1000 xg to pellet cells and remove the supernatant.5. Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.6. Place the cells in the 37°C incubator with 5% CO2.7. Allow incubation for 3-4 days to reach confluence. The cells will re-attach to the surface over a period of several days in culture at 37°C.
Subculturing: 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.5. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Freeze Medium: Complete growth medium 90%; DMSO, 10%
Storage: Liquid nitrogen
Preservation: 1. Detach cells from culture dish according to the Sub-Culture Procedure.2. Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.Note: A T-75 culture flask typically yields enough cells for preparing two frozen vials.3. Aliquot 1 mL cells into cryogenic vials.4. Place vials in a freezing container and store at –80 °C overnight.5. Transfer vials to liquid nitrogen for long term storage. If properly stored, cells should remain stable for years.
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the stable line.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Gene Name: O3FAR1 omega-3 fatty acid receptor 1 [ Homo sapiens ]
Official Symbol: O3FAR1
Synonyms: O3FAR1; omega-3 fatty acid receptor 1; G protein coupled receptor 120 , G protein coupled receptor 129 , GPR120, GPR129; PGR4; G protein-coupled receptor 120; G protein-coupled receptor 129; G-protein coupled receptor 120; G-protein coupled receptor 129; G protein-coupled receptor PGR4; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; G-protein-coupled receptor GT01; GT01; BMIQ10; GPR120; GPR129; MGC119984; DKFZp686F0824;
Gene ID: 338557
mRNA Refseq: NM_001195755
Protein Refseq: NP_001182684
MIM: 609044
UniProt ID: Q5NUL3
Chromosome Location: 10q23.33
Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Free fatty acid receptors, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Regulation of Insulin Secretion, organism-specific biosystem;
Function: fatty acid binding; receptor activity; signal transducer activity; taste receptor activity;
Products Types ◆ Recombinant Protein

Related Gene

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.