"FPR1" Related Products

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Human FPR1-FLAG Stable Cell Line-HEK293T Galphaqi5

Cat.No.: CSC-RG0322
Cell Line Description: HEK293T Galphaqi5-HuFPR1-FLAG cell line is a hypotriploid human cell line, which has been transfected with a human formyl peptide receptor 1 (FPR1) tagged in the N-terminus with FLAG to allow stably express of the human FPR1 tagged in the N-terminus with FLAG. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Background: The gene FPR1 encodes the formylpeptide receptor (FPR), which is a G-protein-coupled receptor that mediates chemotaxis of phagocytic leukocytes induced by bacterial peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP). Agonist binding to FPR in phagocytic leukocytes leads to the activation of phosphatidylinositol 3-kinase (PI3K), mitogen-activated protein kinases (MAPKs), and the transcription factor nuclear factor (NF)-kB via heterotrimeric Galphai proteins. FPR is involved in host defense against bacterial infection and in the clearance of damaged cells. Recently a large number of non-formylated peptide ligands for FPR have now been identified. Some of the new ligands (e.g. Ac1–26 from annexin) are endogenous in origin, and some come from pathogens that are associated with human diseases such as HIV, which have suggested novel roles for this receptor in the regulation of acute and chronic inflammation as well as host responses during HIV-1 infection.
Growth Properties: Adherent
Morphology: Epithelial
Host Cell: HEK293T Galphaqi5
Cell Line Validation: 1. Gene expression: RT-PCR experiments determined specific expression of human FPR1.2. Protein expression: FPR1 expression in this cell line has been validated by WB.3. Functional validation: Functional assays.
Sub-type: Formyl Peptide
Propagation: Complete growth medium: DMEM, 10% FBS, 1 μg/mL puromycin, 150 μg/mL hygromycinAtmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Starting Cells From Frozen Cell Stock: 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37°C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. Clean the outside of the vial with 70% ethanol before opening.3. Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media. 4. Centrifuge for 3 minutes 1000 xg to pellet cells and remove the supernatant.5. Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.6. Place the cells in the 37°C incubator with 5% CO2.7. Allow incubation for 3-4 days to reach confluence. The cells will re-attach to the surface over a period of several days in culture at 37°C.
Subculturing: 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.5. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 weekly is recommended Medium Renewal: 2 to 3 times per week
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Freeze Medium: Complete growth medium 90%; DMSO, 10%
Storage: Liquid nitrogen
Preservation: 1. Detach cells from culture dish according to the Sub-Culture Procedure.2. Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.Note: A T-75 culture flask typically yields enough cells for preparing two frozen vials.3. Aliquot 1 mL cells into cryogenic vials.4. Place vials in a freezing container and store at –80 °C overnight.5. Transfer vials to liquid nitrogen for long term storage. If properly stored, cells should remain stable for years.
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the stable line.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Gene Name: FPR1 formyl peptide receptor 1 [ Homo sapiens ]
Official Symbol: FPR1
Synonyms: FPR1; formyl peptide receptor 1; fMet-Leu-Phe receptor; FMLP; FPR; fMLP receptor; N-formylpeptide chemoattractant receptor;
Gene ID: 2357
mRNA Refseq: NM_001193306
Protein Refseq: NP_001180235
MIM: 136537
UniProt ID: P21462
Chromosome Location: 19q13.41
Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Formyl peptide receptors bind formyl peptides and many other ligands, organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem;
Function: G-protein coupled receptor activity; N-formyl peptide receptor activity; receptor activity; signal transducer activity;

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