"ADRA1A" Related Products

Expand
Expand

Human ADRA1A Stable Cell Line-CHO

Cat.No.: CSC-RG0794
Cell Line Description: CHO-HuADRA1A cell line is clonally-derived from a CHO cell line, which has been transfected with a human adrenoceptor alpha 1A ADRA1A to allow expression of the human ADRA1A. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Background: Alpha-1 adrenergic receptors are widely distributed and mediate a variety of functions including smooth muscle contraction, cardiac stimulation, cellular proliferation, apoptosis and activation of hepatic gluconeogenesis and clycogenolysis. Three distinct alpha-1 adrenergic receptors have been identified and are known as alpha-1a, alpha-1b, and alpha-1c receptors.
Growth Properties: Adherent
Morphology: Epithelial-like
Host Cell: CHO-K1
Cell Line Validation: 1. Gene expression: qPCR experiments determined specific expression of human ADRA1A.2. Protein expression: ADRA1A expression in this cell line has been validated by WB.3. Functional validation: calcium assay.
Stability: 6 passages (4 weeks of continuous culture)
Sub-type: Adrenoceptors
Propagation: Complete growth medium: Ham"s F12, 10% dialyzed FBS, 0.4 mg/mL G418Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Starting Cells From Frozen Cell Stock: 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37°C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. Clean the outside of the vial with 70% ethanol before opening.3. Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media. 4. Centrifuge for 3 minutes 1000 xg to pellet cells and remove the supernatant.5. Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.6. Place the cells in the 37°C incubator with 5% CO2.7. Allow incubation for 3-4 days to reach confluence. The cells will re-attach to the surface over a period of several days in culture at 37°C.
Subculturing: 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.5. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Freeze Medium: Complete growth medium 95%; DMSO, 5%
Storage: Liquid nitrogen
Preservation: 1. Detach cells from culture dish according to the Sub-Culture Procedure.2. Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.Note: A T-75 culture flask typically yields enough cells for preparing two frozen vials.3. Aliquot 1 mL cells into cryogenic vials.4. Place vials in a freezing container and store at –80 °C overnight.5. Transfer vials to liquid nitrogen for long term storage. If properly stored, cells should remain stable for years.
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the stable line.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Gene Name: ADRA1A adrenergic, alpha-1A-, receptor [ Homo sapiens ]
Official Symbol: ADRA1A
Synonyms: ADRA1A; adrenergic, alpha-1A-, receptor; ADRA1C; alpha-1A adrenergic receptor; ADRA1L1; alpha-1A adrenoceptor; alpha-1A adrenoreceptor; G protein coupled receptor; alpha-1C adrenergic receptor; adrenergic, alpha-1A-, receptor variant 1; adrenergic, alpha-1A-, receptor variant 3; adrenergic, alpha-1A-, receptor variant 5; adrenergic, alpha-1A-, receptor variant 8; ALPHA1AAR;
Gene ID: 148
mRNA Refseq: NM_000680
Protein Refseq: NP_000671
MIM: 104221
UniProt ID: P35348
Chromosome Location: 8p21.2
Pathway: Adrenoceptors, organism-specific biosystem; Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (12/13) signalling events, organism-specific biosystem;
Function: G-protein coupled receptor activity; adrenergic receptor activity; alpha1-adrenergic receptor activity; alpha1-adrenergic receptor activity; alpha1-adrenergic receptor activity; receptor activity; signal transducer activity;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.