"CACNA1G" Related Products

Human CACNA1G Stable Cell Line-HEK293

Cat.No.: CSC-RI0077
Cell Line Description: HEK293-HuCACNA1G cell line is a hypotriploid human cell line, which has been transfected with a human calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G) to allow stably express of the human CACNA1G. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Background: The human CACNA1G gene encodes the pore-forming subunit of Cav3.1, a voltage-gated T-type calcium channel.
Growth Properties: Adherent
Morphology: Epithelial
Host Cell: HEK293
Cell Line Validation: 1. Gene expression: qPCR experiments determined specific over-expression of human CACNA1G.2. Protein expression: CACNA1G expression in this cell line has been validated by WB.3. Functional validation: manual patch clamp. Mean Ca2+ current amplitude ± SEM at -30 mV= 1.03 nA ± 0..39, n = 6 cells recorded in manual patch clamp.
Sub-type: Voltage-gated calcium channels
Propagation: Complete growth medium: DMEM/F12; 10% FBSAtmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Starting Cells From Frozen Cell Stock: 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37°C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. Clean the outside of the vial with 70% ethanol before opening.3. Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media. 4. Centrifuge for 3 minutes 1000 xg to pellet cells and remove the supernatant.5. Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.6. Place the cells in the 37°C incubator with 5% CO2.7. Allow incubation for 3-4 days to reach confluence. The cells will re-attach to the surface over a period of several days in culture at 37°C.
Subculturing: 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.5. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: 2 to 3 times per week
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Freeze Medium: Complete growth medium 90%; DMSO, 10%
Storage: Liquid nitrogen
Preservation: 1. Detach cells from culture dish according to the Sub-Culture Procedure.2. Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.Note: A T-75 culture flask typically yields enough cells for preparing two frozen vials.3. Aliquot 1 mL cells into cryogenic vials.4. Place vials in a freezing container and store at –80 °C overnight.5. Transfer vials to liquid nitrogen for long term storage. If properly stored, cells should remain stable for years.
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the stable line.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Gene Name: CACNA1G calcium channel, voltage-dependent, T type, alpha 1G subunit [ Homo sapiens ]
Official Symbol: CACNA1G
Synonyms: CACNA1G; Ca(V)T.1; Cav3.1; NBR13; calcium channel, voltage-dependent, T type, alpha 1G subunit; cav3.1c; voltage-dependent T-type calcium channel subunit alpha-1G; voltage-dependent calcium channel alpha 1G subunit; voltage-gated calcium channel subunit alpha Cav3.1; voltage-dependent calcium channel alpha 1G subunit, isoform 11
Gene ID: 8913
mRNA Refseq: NM_001256324.1
Protein Refseq: NP_001243253.1
MIM: 604065
UniProt ID: O43497
Chromosome Location: 17q22
Pathway: Axon guidance; Calcium signaling pathway; Developmental Biology; MAPK signaling pathway; NCAM signaling for neurite out-growth; NCAM1 interactions; Nicotine Activity on Chromaffin Cells;
Function: low voltage-gated calcium channel activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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