"Mchr1" Related Products

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Rat Mchr1 Stable Cell Line-HEK293T Galphaqi5

Cat.No.: CSC-RG0294
Cell Line Description: HEK293T Galphaqi5-RatMchr1 cell line is a hypotriploid human cell line, which has been transfected with a rat melanin-concentrating hormone receptor 1 (Mchr1) to allow stably express of the rat Mchr1. It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.
Background: MCHR1 or GPR24 is a receptor for melanin-concentrating hormone (MCH). MCH-1R is one of the two MCH receptors identified in human. The primary function of both MCH receptors, MCHR1 and MCHR2, is to regulate skin color. In addition, they also regulate food intake, energy expenditure, behavior and emotion. Blockade of MCHR1 could provide a viable approach for treatment of obesity and certain neurological disorders.
Growth Properties: Adherent
Morphology: Epithelial
Host Cell: HEK293T Galphaqi5
Cell Line Validation: 1. Gene expression: RT-PCR experiments determined specific expression of rat Mchr1.2. Protein expression: Mchr1 expression in this cell line has been validated by WB.3. Functional validation: Functional assays.
Sub-type: Melanin-Concentrating Hormone
Propagation: Complete growth medium: DMEM, 10% FBS, 100μg/mL zeocin, 100μg/mL G418Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Starting Cells From Frozen Cell Stock: 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. Float the cells in the 37°C water bath for 2 minutes until nearly (80%) thawed. Once cells are thawed, it is important to dilute the cells 1:10 in growth media immediately to reduce the potentially toxic effects of the DMSO preservative on the cells.2. Clean the outside of the vial with 70% ethanol before opening.3. Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media. 4. Centrifuge for 3 minutes 1000 xg to pellet cells and remove the supernatant.5. Add 14 ml of media and transfer cells to a T25 flask or a 100 mm culture dish.6. Place the cells in the 37°C incubator with 5% CO2.7. Allow incubation for 3-4 days to reach confluence. The cells will re-attach to the surface over a period of several days in culture at 37°C.
Subculturing: 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.5. Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 weekly is recommended Medium Renewal: 2 to 3 times per week
Mycoplasma: Mycoplasma Status: Negative (MycoAlert Kit)
Freeze Medium: Complete growth medium 90%; DMSO, 10%
Storage: Liquid nitrogen
Preservation: 1. Detach cells from culture dish according to the Sub-Culture Procedure.2. Resuspend cells at a density of 5 x 10^6 cells/mL in freeze medium.Note: A T-75 culture flask typically yields enough cells for preparing two frozen vials.3. Aliquot 1 mL cells into cryogenic vials.4. Place vials in a freezing container and store at –80 °C overnight.5. Transfer vials to liquid nitrogen for long term storage. If properly stored, cells should remain stable for years.
Safety Considerations: The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the stable line.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship: Dry ice
Gene Name: Mchr1 melanin-concentrating hormone receptor 1 [ Rattus norvegicus ]
Official Symbol: Mchr1
Synonyms: MCHR1; melanin-concentrating hormone receptor 1; MCHR; MCH1R; SLC-1; MCH-R1; MCHR-1; MCH receptor 1; G protein-coupled receptor 24; G-protein coupled receptor 24; somatostatin receptor-like protein; Slc1; Gpr24; Mch-1r;
Gene ID: 83567
mRNA Refseq: NM_031758
Protein Refseq: NP_113946
Pathway: G alpha (i) signalling events, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem; Signal Transduction, organism-specific biosystem;
Function: G-protein coupled receptor activity; hormone binding; melanin-concentrating hormone receptor activity; melanin-concentrating hormone receptor activity; protein C-terminus binding; receptor activity; signal transducer activity;

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C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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