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Recombinant Human YOD1, His-tagged

Cat.No. : YOD1-7575H
Product Overview : Recombinant human YOD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
  • Specification
  • Gene Information
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Antigen Description : YOD1 is a highly conserved deubiquitinating enzyme of the ovarian tumor (otubain) family, whose function has yet to be assigned in mammalian cells. YOD1 is a constituent of a multiprotein complex with p97 as its nucleus, suggesting a functional link to a pathway responsible for the dislocation of misfolded proteins from the endoplasmic reticulum. Expression of a YOD1 variant deprived of its deubiquitinating activity imposes a halt on the dislocation reaction, as judged by the stabilization of various dislocation substrates.
Source : E. coli
Species : Human
Tag : His
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Molecular Mass : 40.7 kDa (371aa) confirmed by MALDI-TOF
Protein length : 1-348aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSMFGPAKG RHFGVHPAPG FPGGVSQQAA GTKAGPAGAW PVGSRTDTMW RLRCKAKDGT HVLQGLSSRT RVRELQGQIA AITGIAPGGQ RILVGYPPEC LDLSNGDTIL EDLPIQSGDM LIIEEDQTRP RSSPAFTKRG ASSYVRETLP VLTRTVVPAD NSCLFTSVYY VVEGGVLNPA CAPEMRRLIA QIVASDPDFY SEAILGKTNQ EYCDWIKRDD TWGGAIEISI LSKFYQCEIC VVDTQTVRID RFGEDAGYTK RVLLIYDGIH YDPLQRNFPD PDTPPLTIFS SNDDIVLVQA LELADEARRR RQFTDVNRFT LRCMVCQKGL TGQAEAREHA KETGHTNFGE V
Purity : >90% as determined by SDS - PAGE
Applications : SDS-PAGE
Storage : Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration : 0.25 mg/ml
Gene Name : YOD1?YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) [?Homo sapiens?]
Official Symbol : YOD1
Synonyms : YOD1; DUBA8; OTUD2; PRO0907; RP11-164O23.1; ubiquitin thioesterase OTU1; HIN-7; DUBA-8; hsHIN7; OTU domain containing 2; OTU domain containing 2; OTU domain-containing protein 2; YOD1 OTU deubiquinating enzyme 1 homolog ( yeast); EC 3.1.2; YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae); EC 3.4.19.12
Gene ID : 55432
mRNA Refseq : NM_018566
Protein Refseq : NP_061036
MIM : 612023
UniProt ID : Q5VVQ6
Chromosome Location : 1q32.2
Pathway : Protein processing in endoplasmic reticulum
Function : protein binding; ubiquitin-specific protease activity; zinc ion binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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