Active Recombinant Mouse Csf1 protein

• Cat.No.: Csf1-290M
• Product Overview: Recombinant Mouse Csf1 was expressed in E. coli.

Specification

• Description: M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells.
• Source: E. coli
• Species: Mouse
• Form: Sterile filtered through a 0.2 micron filter. Lyophilized from 5mM Sodium, Phosphate, pH 8.0 + 100mM NaCl.
• Bio-activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells is < 1.0 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg.
• Molecular Mass: 36.4 kDa
• Protein length: 156 amino acid
• AA Sequence: MKEVSEHCS HMIGNGHLK VLQQLIDSQ METSCQIAF EFVDQEQLD DPVCYLKKA FFLVQDIID ETMRFKDNT PNANATERL QELSNNLNS CFTKDYEEQ NKACVRTFH ETPLQLLEK IKNFFNETK NLLEKDWNI FTKNCNNSF AKCSSRDVV TKP
• Endotoxin: < 0.10 ng/ug of protein(< 1 EU/ug)
• Purity: Above 98% as determined by SDS-PAGE Analysis.
• Storage: Store product at –20 centigrade.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 centigrade to -80 centigrade.

Gene Information

• Gene Name: Csf1 colony stimulating factor 1 (macrophage) [ Mus musculus ]
• Official Symbol: Csf1
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; osteopetrosis; op; Csfm; MCSF; C87615
• Gene ID: 12977
• mRNA Refseq: NM_001113529
• Protein Refseq: NP_001107001
• UniProt ID: P07141
• Chromosome Location: 3 46.83 cM; 3 F3
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response (Bi centigradearta), organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem
• Function: cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; protein binding; protein homodimerization activity; protein homodimerization activity