Recombinant Human Heat Shock 70kDa Protein 5 (glucose-regulated protein, 78kDa), His-tagged

• Cat.No.: HSPA5-4710H
• Product Overview: HSPA5 produced in Hi-5 cells is a single, glycosylated polypeptide chain containing 640 amino acids (20-650 a.a.) and having a molecular mass of 71kDa. HSPA5 is fused to an 8 amino acid His Tag at C-Terminus and purified by proprietary chromatographic techniques.

Specification

• Cat. No.: HSPA5-4710H
• Description: Binding immunoglobulin protein (BiP or HSPA5) is a member of the family of ~70kDa heat shock proteins (HSP 70). HSPA5 is a stress response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. HSPA5 is crucial for the proper glycosylation, folding as well as for the maintenance of cell homeostasis and the prevention of apoptosis.
• Source: Human
• Host: His-5 Cells.
• Form: The HSPA5 protein solution (1mg/ml) 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT and 200mM NaCl.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Physical Appearance: Sterile filtered colorless solution.
• Amino acid sequence: MEEDKKEDVG TVVGIDLGTT YSCVGVFKNG RVEIIANDQG NRITPSYVAF TPEGERLIGD AAKNQLTSNP ENTVFDAKRL IGRTWNDPSV QQDIKFLPFK VVEKKTKPYI QVDIGGGQTK TFAPEEISAM VLTKMKETAE AYLGKKVTHA VVTVPAYFND AQRQATKDAG TIAGLNVMRI INEPTAAAIA YGLDKREGEK NILVFDLGGG TFDVSLLTID NGVFEVVATN GDTHLGGEDF DQRVMEHFIK LYKKKTGKDV RKDNRAVQKL RREVEKAKRA LSSQHQARIE IESFYEGEDF SETLTRAKFE ELNMDLFRST MKPVQKVLED SDLKKSDIDE IVLVGGSTRI PKIQQLVKEF FNGKEPSRGI NPDEAVAYGA AVQAGVLSGD QDTGDLVLLD VCPLTLGIET VGGVMTKLIP RNTVVPTKKS QIFSTASDNQ PTVTIKVYEG ERPLTKDNHL LGTFDLTGIP PAPRGVPQIE VTFEIDVNGI LRVTAEDKGT GNKNKITITN DQNRLTPEEI ERMVNDAEKF AEEDKKLKER IDTRNELESY AYSLKNQIGD KEKLGGKLSS EDKETMEKAV EEKIEWLESH QDADIEDFKA KKKELEEIVQ PIISKLYGSA GPPPTGEEDT AELEHHHHHH.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
• Pathways: Activation of Chaperones by ATF6-alpha; Adaptive Immunity Signaling; Antigen Presentation: Folding, assembly and peptide loading of class I MHC; Antigen processing and presentation; Class I MHC mediated antigen processing & presentation; Diabetes pathways; Formation of Platelet plug; Hemostasis; Immune System; MAPK signaling pathway; PERK regulated gene expression; Platelet Activation; Platelet degranulation; Prion diseases; Unfolded Protein Response

Gene Information

• Gene Name: HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) [ Homo sapiens ]
• Official Symbol: HSPA5
• Synonyms: HSPA5; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); BIP; MIF2; GRP78; GRP 78; FLJ26106; BiP; OTTHUMP00000022124; Heat shock 70 kDa protein 5; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD); Immunoglobulin heavy chain-binding protein; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5
• Gene ID: 3309
• mRNA Refseq: NM_005347
• Protein Refseq: NP_005338
• MIM: 138120
• UniProt ID: P11021
• Chromosome Location: 9q33-q34.1
• Function: ATP binding; calcium ion binding; caspase inhibitor activity; nucleotide binding; protein binding, bridging; ribosome binding; unfolded protein binding