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Active Recombinant Mouse Igfbp6 protein(Met1-Gly238), His-tagged

Cat.No. : Igfbp6-4031M
Product Overview : Recombinant Mouse IGFBP6 (NP_032370.2) (Met 1-Gly 238) was expressed in HEK293, with a polyhistidine tag at the C-terminus.
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Source : HEK293
Species : Mouse
Tag : C-His
Protein length : Met1-Gly238
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 1-4 μg/ml in the presence of 20 ng/mL mouse IGFII.
Molecular Mass : The recombinant mouse IGFBP6 consists of 224 amino acids and has a predicted molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmIGFBP6 is approximately 30 kDa due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : > 92 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Igfbp6 insulin-like growth factor binding protein 6 [ Mus musculus ]
Official Symbol : Igfbp6
Synonyms : IGFBP6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6; IBP-6; IGF-binding protein 6; INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 6 PRECURSOR (IGFBP-6) (IBP-6) (IGF-BINDING PROTEIN 6); IGFBP-6;
Gene ID : 16012
mRNA Refseq : NM_008344
Protein Refseq : NP_032370

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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