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| Cat. No.: | IL4-411R |
| Product Overview: | Interleukin-4 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 126 amino acids and having a molecular mass of 14105 Dalton. The IL-4 is purified by proprietary chromatographic techniques. |
| Description: | IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Source: | Rat |
| Host: | Escherichia Coli. |
| Form: | Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. |
| Purity: | Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Bio-activity: | The ED50 as determined by the dose-dependant prolifiration of Con A activated rat spleen cells is < 1.5 ng/ml, corresponding to a Specific Activity of 660,000 IU/mg. |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Solubility: | It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Amino acid sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Gly-Cys-Asn. |
| Storage: | Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Gene Name: | Il4 interleukin 4 [ Rattus norvegicus ] |
| Official Symbol: | IL4 |
| Synonyms: | interleukin-4; IL-4; BSF-1; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IGG differentiation factor |
| Gene ID: | 287287 |
| mRNA Refseq: | NM_201270 |
| Protein Refseq: | NP_958427 |
| UniProt ID: | P20096 |
| Chromosome Location: | 10q22 |
| Pathway: | Allograft rejection; Autoimmune thyroid disease; Fc epsilon RI signaling pathway; Intestinal immune network for IgA production; Jak-STAT signaling pathway |
| Function: | cytokine activity; growth factor activity; interleukin-4 receptor binding |
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