Recombinant Human Interleukin 6 (Interferon, Beta 2)

Cat. No.: IL6-471H
Product Overview: Recombinant Human IL-6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21,000 Daltons.
Description: Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contains four conserved cysteine residues involved in two disulphide bonds. They have a compact, globular fold (similar to other interleukins), stabilised by the 2 disulphide bonds.
Species: Human
Source: E.coli
Form: Lyophilized from 10 mM Acetic acid.
Purity: Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity: Human IL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of mouse 7TD1 cells is less then 0.08 ng/ml.
Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 10 mM HCl at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK
Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Gene Name: IL6 interleukin 6 (interferon, beta 2) [ Homo sapiens ]
Official Symbol: IL6
Synonyms: HGF; BSF2; HSF; IFNB2; IL-6; CDF; BSF-2; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2; Interleukin-6 precursor; interleukin 6 (interferon, beta 2); IL6
Gene ID: 3569
mRNA Refseq: NM_000600
Protein Refseq: NP_000591
MIM: 147620
UniProt ID: P05231
Chromosome Location: 7p21
Pathway: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Prion disease, Toll-like receptor signaling pathway
Function: Cytokine activity, Interleukin-6 receptor binding, Protein binding

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.