Active Recombinant Mouse Kirrel3 protein(Met1-Ala535), His-tagged

• Cat.No.: Kirrel3-4028M
• Product Overview: Recombinant Mouse KIRREL3 (Q8BR86-1)(Met 1-Ala 535) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Ala535
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to mouse KIRREL3 coated plates (15 μg/mL, 100 μL/well), > 40% will adhere specifically after 90 minutes at 37 °C.
• Molecular Mass: The secreted recombinant mouse KIRREL3 consists of 525 amino acids (pro form) and has a calculated molecular mass of 57.5 kDa. The apparent molecular mass of the recombinant mouse KIRREL3 is approximately 65 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Kirrel3 kin of IRRE like 3 (Drosophila) [ Mus musculus ]
• Official Symbol: Kirrel3
• Synonyms: KIRREL3; kin of IRRE like 3 (Drosophila); kin of IRRE-like protein 3; X kin of IRRE like 3; membrane protein mKirre; kin of irregular chiasm-like protein 3; SST4; NEPH2; mKIAA1867; 1500010O20Rik; 2900036G11Rik
• Gene ID: 67703
• mRNA Refseq: NM_001190911
• Protein Refseq: NP_001177840