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Recombinant Human KITLG (26-189aa), Met tagged

Cat. No.: KITLG-036E
Product Overview: Recombinant Human KITLG (Glu26 ­ Ala189, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.
Description: Stem cell factor (SCF), also known as c­kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 ­ 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
Source: E. coli
N­terminal Sequence Analysi: Met
Molecular Mass: 18.6 kDa
Activity: Measured in a cell proliferation assay using TF­1 human erythroleukemic cells. The ED50 for this effect is typically 1 ­ 5 ng/mL.
Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. See Certificate of Analysis for details.
Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
Unitprot ID: P21583
Gene Name: KITLG KIT ligand [ Homo sapiens ]
Official Symbol: KITLG
Synonyms: KITLG; KIT ligand; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7; kit-ligand; DKFZp686F2250; c-Kit ligand; steel factor; stem cell factor; mast cell growth factor; familial progressive hyperpigmentation 2
Gene ID: 4254
mRNA Refseq: NM_003994
Protein Refseq: NP_003985
MIM: 184745
Chromosome Location: 12q22
Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Kit Receptor Signaling Pathway; Melanogenesis; Pathways in cancer
Function: cytokine activity; growth factor activity; protein binding; stem cell factor receptor binding

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