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Active Recombinant Human Kallikrein-Related Peptidase 11

Cat.No. : KLK11-1360H
Product Overview : KLK11 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain having a molecular weight of 25.6kDa though on SDS-PAGE migrates at about 41kDa due to the glycosilation. The KLK11 is purified by proprietary chromatographic techniques.
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Cat. No. : KLK11-1360H
Description : KLK11 is a multifunctional protease. KLK11 cleaves "bz-Phe-Arg-4-methylcoumaryl-7-amide", KLK11 is a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. KLK11 cleaves synthetic peptides after arginine rather than lysine residues.
Source : Human
Host : HEK293-F Cells.
Form : The KLK11 protein was lyophilized from a 0.2µm filtered solution containing no additives or preservatives.
Purity : Greater than 95.0% as determined by SDS-PAGE.
Bio-activity : KLK11 specific activity is greater than 2200 pmoles/min/μg when measure with 100uM colormetric peptide substrate (D-Val-Leu-Lys-ThioBenzyl ester) and 1 μg of activated enzyme. The reaction is carried out in a volume of 100μL containing 50mM Tris, 1M NaCl, 10mM EDTA, 0.1mM DTNB (5,5μDithio-bis(2-nitrobenzoic acid)), pH8.5 at 37°C. Cleavage of the peptide substrate can be measured at a wavelength of 405nm and quantified using the extinction coeffiecient 13,260M-1cm-1.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility : It is recommended to reconstitute the lyophilized KLK11 in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino acid sequence : ETRIIKGFEC KPHSQPWQAA LFEKTRLLCG ATLIAPRWLL TAAHCLKPRY IVHLGQHNLQ KEEGCEQTRT ATESFPHPGF NNSLPNKDHR NDIMLVKMAS PVSITWAVRP LTLSSRCVTA GTSCLISGWG STSSPQLRLP HTLRCANITI IEHQKCENAY PGNITDTMVC ASVQEGGKDS CQGDSGGPLV CNQSLQGIIS WGQDPCAITR KPGVYTKVCK YVDWIQETMK NN.
Storage : Lyophilized KLK11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KLK11 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name : KLK11 kallikrein-related peptidase 11 [ Homo sapiens ]
Official Symbol : KLK11
Synonyms : kallikrein-related peptidase 11; TLSP; PRSS20; MGC33060; KLK11; hK11; kallikrein 11; hippostasin; protease, serine, trypsin-like; protease, serine, 20 trypsin-like; EC 3.4.21.-; Hippostasin; hippostasin; Serine protease 20; Trypsin-like protease; kallikrein-related peptidase 11
Gene ID : 11012
mRNA Refseq : NM_001136032
Protein Refseq : NP_001129504
MIM : 604434
UniProt ID : Q9UBX7
Chromosome Location : 19q13.3-q13.4
Function : serine-type endopeptidase activity; peptidase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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