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Recombinant Human PKLR, His-tagged

Cat. No.: PKLR-1517H
Product Overview: Recombinant human PKLR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (47-574 aa).
Description: PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. The PKLR gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters.
Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol
Molecular Weight: 59.2 kDa (549aa), confirmed by MALDI-TOF
Purity: > 90% by SDS - PAGE
Concentration: 1 mg/ml (determined by Bradford assay)
Biological activity: Specific activity: >0.1 unit/mg. One unit will form 1.0 umol of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C
Activity Assay: 1. Prepare a 1.45 ml reaction mixture into a suitable container. - Reaction mixture: 100mM Tris-HCl pH 7.5, 7.6 mM ADP, 15 mM MgCl2, 74 mM KCl, 0.2 mM Beta-NADHμ 5.2 mM PEP, 0.025 units Recombinant LDHA protein. 2. Add 50ul of recombinant PKLR protein solution with various concentrations (0.5ug, 1ug). 3. Read the decrease in A340nm in kinetic mode for 10 minutes.
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways: Glycolysis / Gluconeogenesis; Insulin signaling pathway; Maturity onset diabetes of the young; Metabolic pathways; Purine metabolism; Pyruvate metabolism; Type II diabetes mellitus
Gene Name: PKLR pyruvate kinase, liver and RBC [ Homo sapiens ]
Official Symbol: PKLR
Synonyms: PKLR; pyruvate kinase, liver and RBC; PK1; PKL; PKR; RPK; PKRL; pyruvate kinase isozymes R/L; pyruvate kinase 1; OTTHUMP00000034069; pyruvate kinase type L; pyruvate kinase isozyme R/L; R-type/L-type pyruvate kinase; red cell/liver pyruvate kinase; pyruvate kinase, liver and blood cell; EC 2.7.1.40
Gene ID: 5313
mRNA Refseq: NM_000298
Protein Refseq: NP_000289
MIM: 609712
UniProt ID: P30613
Chromosome Location: 1q21
Function: ATP binding; magnesium ion binding; nucleotide binding; potassium ion binding; pyruvate kinase activity; transferase activity

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