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Recombinant Human Peptidylprolyl Isomerase F, His-tagged

Cat. No.: PPIF-1429H
Product Overview: PPIF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 198 amino acids (30-207) and having a molecular mass of 21 kDa. The PPIF is fused to 20 amino acid His tag at N-terminus and is purified by proprietary chromatographic techniques.
Description: PPIF is a part of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIF accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is key component of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
Source: Human
Host: Escherichia Coli.
Form: PPIF solution containing 20 mM Tris pH-7.5, 10% glycerol and 1 mM DTT.
Purity: Greater than 95.0% as determined by SDS-PAGE.
Bio-activity: Specific activity is > 120 nmoles/min/ug, and is defined as the amount of enzyme that cleave 1 umole of suc-AAFP-pNA per minute at 1C in Tris-Hcl pH8.0 using chymotrypsin.
Physical Appearance: Sterile filtered colorless solution.
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH CSKGSGDPSS SSSSGNPLVY LDVDANGKPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FTLKHVGPGV LSMANAGPNT NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGRTSKKIV ITDCGQLS
Storage: PPIF Human Recombinant although stable at 4°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.
Gene Name: PPIF peptidylprolyl isomerase F [ Homo sapiens ]
Official Symbol: PPIF
Synonyms: PPIF; peptidylprolyl isomerase F; CYP3; Cyp-D; FLJ90798; MGC117207; peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F; rotamase F; cyclophilin 3; cyclophilin D; cyclophilin F; OTTHUMP00000019925; OTTHUMP00000019926; peptidyl-prolyl cis-trans isomerase, mitochondrial
Gene ID: 10105
mRNA Refseq: NM_005729
Protein Refseq: NP_005720
MIM: 604486
UniProt ID: P30405
Chromosome Location: 10q22-q23
Pathway: Toxoplasmosis
Function: cyclosporin A binding; isomerase activity; peptidyl-prolyl cis-trans isomerase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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