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Active Recombinant Human PRSS3 protein, His-tagged

Cat.No. : PRSS3-1170H
Product Overview : Recombinant Human PRSS3 protein(P35030-3)(Met1-Ser247), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile 50mM MES, 0.6M NaCl, pH 5.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >4,000 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)
Molecular Mass : The secreted recombinant pro form of the human PRSS3 consists of 243 amino acids and predictes a molecular mass of 26.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPRSS3 is approximately 33 kDa due to glycosylation.
Protein Length : Met1-Ser247
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : PRSS3 protease, serine, 3 [ Homo sapiens ]
Official Symbol : PRSS3
Synonyms : PRSS3; protease, serine, 3; protease, serine, 3 (mesotrypsin) , protease, serine, 4 (trypsin 4, brain) , PRSS4; trypsin-3; mesotrypsin; TRY3; TRY4; trypsin IV; trypsin III; trypsinogen 4; trypsinogen 5; trypsinogen IV; mesotrypsinogen; brain trypsinogen; pancreatic trypsinogen III; protease, serine, 4 (trypsin 4, brain); T9; MTG; PRSS4;
Gene ID : 5646
mRNA Refseq : NM_001197097
Protein Refseq : NP_001184026
MIM : 613578
UniProt ID : P35030

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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