||Recombinant human PSMA6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||Proteasome subunit alpha type 6, also known as PSMA6, is a member of the peptidase T1A family, which is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. PSMA6 is distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1M NaCl
||29.9 kDa (270aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.25 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMSRGSS AGFDRHITIF SPEGRLYQVE YAFKAINQGG LTSVAVRGKD CAVIVTQKKV PDKLLDSSTV THLFKITENI GCVMTGMTAD SRSQVQRARY EAANWKYKYG YEIPVDMLCK RIADISQVYT QNAEMRPLGC CMILIGIDEE QGPQVYKCDP AGYYCGFKAT AAGVKQTEST SFLEKKVKKK FDWTFEQTVE TAITCLSTVL SIDFKPSEIE VGVVTVENPK FRILTEAEID AHLVALAERD
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||APC/C-mediated degradation of cell cycle proteins; Adaptive Immunity Signaling; Apoptosis; Assembly of the pre-replicative complex; Autodegradation of Cdh1 by Cdh1:APC/C; Autodegradation of the E3 ubiquitin ligase COP1; Cell Cycle Checkpoints; Cell Cycle, Mitotic; Cyclin A:Cdk2-associated events at S phase entry; DNA Replication Pre-Initiation; G1/S DNA Damage Checkpoints; G1/S Transition; HIV Infection; Host Interactions of HIV factors; Immune System