Recombinant Human Proteasome (Prosome, Macropain) Subunit, Alpha Type, 6, His-tagged

• Cat.No.: PSMA6-4749H
• Product Overview: Recombinant human PSMA6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: PSMA6-4749H
• Description: Proteasome subunit alpha type 6, also known as PSMA6, is a member of the peptidase T1A family, which is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. PSMA6 is distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1M NaCl
• Molecular Weight: 29.9 kDa (270aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMSRGSS AGFDRHITIF SPEGRLYQVE YAFKAINQGG LTSVAVRGKD CAVIVTQKKV PDKLLDSSTV THLFKITENI GCVMTGMTAD SRSQVQRARY EAANWKYKYG YEIPVDMLCK RIADISQVYT QNAEMRPLGC CMILIGIDEE QGPQVYKCDP AGYYCGFKAT AAGVKQTEST SFLEKKVKKK FDWTFEQTVE TAITCLSTVL SIDFKPSEIE VGVVTVENPK FRILTEAEID AHLVALAERD
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: APC/C-mediated degradation of cell cycle proteins; Adaptive Immunity Signaling; Apoptosis; Assembly of the pre-replicative complex; Autodegradation of Cdh1 by Cdh1:APC/C; Autodegradation of the E3 ubiquitin ligase COP1; Cell Cycle Checkpoints; Cell Cycle, Mitotic; Cyclin A:Cdk2-associated events at S phase entry; DNA Replication Pre-Initiation; G1/S DNA Damage Checkpoints; G1/S Transition; HIV Infection; Host Interactions of HIV factors; Immune System

Gene Information

• Gene Name: PSMA6 proteasome (prosome, macropain) subunit, alpha type, 6 [ Homo sapiens ]
• Official Symbol: PSMA6
• Synonyms: PSMA6; IOTA; p27K; PROS27; MGC2333; MGC22756; MGC23846; proteasome subunit alpha type-6; PROS-27; OTTHUMP00000178843; macropain iota chain; prosomal P27K protein; proteasome iota chain; macropain subunit iota; 27 kDa prosomal protein; proteasome subunit iota; multicatalytic endopeptidase complex iota chain; proteasome (prosome, macropain) subunit, alpha type, 6; Macropain iota chain; Multicatalytic endopeptidase complex iota chain; Proteasome iota chain; EC 3.4.25.1; P27K
• Gene ID: 5687
• mRNA Refseq: NM_002791
• Protein Refseq: NP_002782
• MIM: 602855
• UniProt ID: P60900
• Chromosome Location: 14q13
• Function: NF-kappaB binding; RNA binding; endopeptidase activity; peptidase activity; protein binding; purine ribonucleoside triphosphate binding; threonine-type endopeptidase activity