||Recombinant human PSMB3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl
||25.1 kDa (225aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.25 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MSIMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP DHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL KARMD
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||APC/C-mediated degradation of cell cycle proteins; APC/C:Cdc20 mediated degradation of Securin; Adaptive Immunity Signaling; Apoptosis; Assembly of the pre-replicative complex; Autodegradation of the E3 ubiquitin ligase COP1; CDK-mediated phosphorylation and removal of Cdc6; CDT1 association with the CDC6:ORC:origin complex; Cell Cycle Checkpoints; Cell Cycle, Mitotic; Cyclin A:Cdk2-associated events at S phase entry; Cyclin E associated events during G1/S transition; Vpu mediated degradation of CD4; Vif-mediated degradation of APOBEC3G; Synthesis of DNA