||Recombinant human RBBP9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||Retinoblastoma binding protein, also known as RBBP9, localizes to both the nucleus and the cytoplasm. RBBP9 is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. Specifically, RBBP9 interacts with retinoblastoma(Rb) and provides cellular resistance to the growth-inhibitory effects of TGFbeta1, thus facilitating the cellular transformation process.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
||23.1 kDa (206aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MASPSKAVIV PGNGGGDVTT HGWYGWVKKE LEKIPGFQCL AKNMPDPITA RESIWLPFME TELHCDEKTI IIGHSSGAIA AMRYAETHRV YAIVLVSAYT SDLGDENERA SGYFTRPWQW EKIKANCPYI VQFGSTDDPF LPWKEQQEVA DRLETKLHKF TDCGHFQNTE FHELITVVKS LLKVPA
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.