||Recombinant human RPS7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||RPS7 belongs to the S3P family of ribosomal proteins and is located in the cytoplasm. RPS7 may play a role in ribosomal stress, linking ribosome biogenesis to cell death or cell cycle arrest. Also, RPS7 interacts with MDM2 and is believed to negatively regulate the MDM2-mediated degradation of p53.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT
||24.7 kDa (218aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSHMFSSSA KIVKPNGEKP DEFESGISQA LLELEMNSDL KAQLRELNIT AAKEIEVGGG RKAIIIFVPV PQLKSFQKIQ VRLVRELEKK FSGKHVVFIA QRRILPKPTR KSRTKNKQKR PRSRTLTAVH DAILEDLVFP SEIVGKRIRV KLDGSRLIKV HLDKAQQNNV EHKVETFSGV YKKLTGKDVN FEFPEFQL
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Cap-dependent Translation Initiation; Cytoplasmic Ribosomal Proteins; Diabetes pathways; Eukaryotic Translation Elongation; Eukaryotic Translation Initiation; Eukaryotic Translation Termination; Formation of a pool of free 40S subunits; Gene Expression; Influenza Infection; Influenza Life Cycle; Peptide chain elongation; Ribosome; Translation; Viral mRNA Translation