Recombinant Human SIRT1 protein(Met193-Ser747), His-tagged

• Cat.No.: SIRT1-1165H
• Product Overview: Recombinant Human SIRT1 (Met 193-Ser 747) was expressed in E. coli with a polyhistide tag at the N-terminus.

Specification

• Source: E. coli
• Species: Human
• Tag: N-His
• Protein length: Met193-Ser747
• Form: Lyophilized from sterile PBS, pH 7.4, 10% glycerol Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human SIRT1 consists of 562 amino acids and has a calculated molecular mass of 62.8 kDa.
• Purity: > 65 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SIRT1 sirtuin 1 [ Homo sapiens ]
• Official Symbol: SIRT1
• Synonyms: SIRT1; sirtuin 1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; NAD-dependent deacetylase sirtuin-1; SIR2L1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1
• Gene ID: 23411
• mRNA Refseq: NM_001142498
• Protein Refseq: NP_001135970
• MIM: 604479
• UniProt ID: Q96EB6