||Recombinant human SUMO1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||SUMO1, also known as small ubiquitin-related modifier 1, is a member of the SUMO protein family and functions in a manner similar to ubiquitin. However, unlike ubiquitin which targets proteins for degradation, SUMO1 protein participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability.
||Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
||12.6 kDa (109aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
||> 90 % by SDS - PAGE
||1 mg/ml (determined by Bradford)
|Sequences of amino acids:
||MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGGHST VLEHHHHHH
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.