Recombinant Human Thiosulfate Sulfurtransferase (rhodanese), His-tagged

• Cat.No.: TST-1456H
• Product Overview: Recombinant Human TST produced in E.Coli is a single, non-glycosylated polypeptide chain containing 317 amino acids (1-297 a.a) and having a molecular mass of 35.6 kDa. TST is fused to a 20 amino acid His-Tag at N-terminus and purified by conventional chromatography techniques.

Specification

• Cat. No.: TST-1456H
• Description: TST is a mitochondrial matrix enzyme that is encoded by the nucleus. TST is involved in cyanide detoxification, the formation of iron-sulfur proteins, and the modification of sulfur-containing enzymes. TST catalyzes the chemical reaction of thiosulfate & cyanide to sulfite & thiocyanate (detoxification). TST is part of the transferase family of proteins. TST includes two highly conservative domains, identified as rhodanese homology domains. In mammals, the majority of cyanide is converted to thiocyanate. TST has weak mercaptopyruvate sulfurtransferase activity.
• Source: Human
• Host: Escherichia Coli
• Form: The Rhodanese protein solution contains 20mM Tris-HCl, pH-8 and 10% Glycerol.
• Purity: Greater than 95.0% as determined by SDS-PAGE
• Physical Appearance: Sterile filtered colorless solution.
• Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MVHQVLYRAL VSTKWLAESI RTGKLGPGLR VLDASWYSPG TREARKEYLE RHVPGASFFD IEECRDTASP YEMMLPSEAG FAEYVGRLGI SNHTHVVVYD GEHLGSFYAP RVWWMFRVFG HRTVSVLNGG FRNWLKEGHP VTSEPSRPEP AVFKATLDRS LLKTYEQVLE NLESKRFQLV DSRSQGRFLG TEPEPDAVGL DSGHIRGAVN MPFMDFLTED GFEKGPEELR ALFQTKKVDL SQPLIATCRK GVTACHVALA AYLCGKPDVA VYDGSWSEWF RRAPPESRVS QGKSEKA.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Gene Information

• Gene Name: TST thiosulfate sulfurtransferase (rhodanese) [ Homo sapiens ]
• Official Symbol: TST
• Synonyms: TST; thiosulfate sulfurtransferase (rhodanese); RDS; MGC19578; thiosulfate sulfurtransferase; OTTHUMP00000197761
• Gene ID: 7263
• mRNA Refseq: NM_003312
• Protein Refseq: NP_003303
• MIM: 180370
• UniProt ID: Q16762
• Chromosome Location: 22q13
• Pathway: Cysteine and methionine metabolism; Sulfur relay system; 5S rRNA binding
• Function: thiosulfate sulfurtransferase activity; transferase activity; transferase activity