||Recombinant human TUBG1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
||TUBG1 is a member of the tubulin superfamily. This protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. It mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle.
||Liquid. In 20mM Tris-HCl buffer(pH 8.0) 1M Urea, 5% Glycerol.
||53.3 kDa (471aa)
||> 85 % by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MPREIITLQL GQCGNQIGFE FWKQLCAEHG ISPEGIVEEF ATEGTDRKDV FFYQADDEHY IPRAVLLDLE PRVIHSILNS PYAKLYNPEN IYLSEHGGGA GNNWASGFSQ GEKIHEDIFD IIDREADGSD SLEGFVLCHS IAGGTGSGLG SYLLERLNDR YPKKLVQTYS VFPNQDEMSD VVVQPYNSLL TLKRLTQNAD CVVVLDNTAL NRIATDRLHI QNPSFSQINQ LVSTIMSAST TTLRYPGYMN NDLIGLIASL IPTPRLHFLM TGYTPLTTDQ SVASVRKTTV LDVMRRLLQP KNVMVSTGRD RQTNHCYIAI LNIIQGEVDP TQVHKSLQRI RERKLANFIP WGPASIQVAL SRKSPYLPSA HRVSGLMMAN HTSISSLFER TCRQYDKLRK REAFLEQFRK EDMFKDNFDE MDTSREIVQQ LIDEYHAATR PDYISWGTQE Q
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Cell Cycle, Mitotic; Centrosome maturation; G2/M Transition; Loss of Nlp from mitotic centrosomes; Loss of proteins required for interphase microtubule organizationÂ from the centrosome; Mitotic G2-G2/M phases; PLK1 signaling events; Recruitment of NuMA to mitotic centrosomes; Recruitment of mitotic centrosome proteins and complexes