"Vegfa" Related Products

Recombinant Mouse Vascular Endothelial Growth Factor A

Cat. No.: Vegfa-585M
Product Overview: Recombinant Mouse VEGF produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 39,035 Daltons.
Description: Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1 , FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF.
Species: Mouse
Source: E.coli
Form: Lyophilized with no additives.
Purity: Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity: The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-5.0 ng/ml.
Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility: It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP
Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathways: Bladder cancer; Focal adhesion; Cytokine-cytokine receptor interaction; Pancreatic cancer; Pathways in cancer; Renal cell carcinoma; VEGF signaling pathway; mTOR signaling pathway
Gene Name: Vegfa vascular endothelial growth factor A [ Mus musculus ]
Official Symbol: Vegfa
Synonyms: Vegfa; vascular endothelial growth factor A;Vpf; Vegf; Vegf-a; Vegf120; Vegf164; VEGF120; VPF; Vegf188; OTTMUSP00000017463; VEGF164; OTTMUSP00000017464; VEGF-A; OTTMUSP00000022243; vascular permeability factor; VEGF188; MGC70609; MVCD1; VEGF; Vascular permeability factor; vascular endothelial growth factor; vascular endothelial growth factor isoform VEGF165
Gene ID: 22339
mRNA Refseq: NM_001025250
Protein Refseq: NP_001020421
UniProt ID: Q00731
Chromosome Location: 17 C; 17 24.2 cM
Function: growth factor activity; heparin binding

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