"Vegfa" Related Products

Recombinant Mouse Vascular Endothelial Growth Factor A

Cat. No. : Vegfa-585M
Product Overview : Recombinant Mouse VEGF produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 39,035 Daltons.
Description : Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as Tumor Angiogenesis Factor or Vascular Permeability Factor. Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. Alternative splicing of the mRNA for VEGF-A results in several isoforms of the protein being produced. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 percent. In contrast to other factors mitogenic for endothelial cells such as FGF-1 , FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF.
Species : Mouse
Source : E.coli
Form : Lyophilized with no additives.
Purity : Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity : The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-5.0 ng/ml.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 95% as determined by : Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by : UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level : Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence : MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP
Storage : Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathways : Bladder cancer; Focal adhesion; Cytokine-cytokine receptor interaction; Pancreatic cancer; Pathways in cancer; Renal cell carcinoma; VEGF signaling pathway; mTOR signaling pathway
Gene Name : Vegfa vascular endothelial growth factor A [ Mus musculus ]
Official Symbol : Vegfa
Synonyms : Vegfa; vascular endothelial growth factor A;Vpf; Vegf; Vegf-a; Vegf120; Vegf164; VEGF120; VPF; Vegf188; OTTMUSP00000017463; VEGF164; OTTMUSP00000017464; VEGF-A; OTTMUSP00000022243; vascular permeability factor; VEGF188; MGC70609; MVCD1; VEGF; Vascular permeability factor; vascular endothelial growth factor; vascular endothelial growth factor isoform VEGF165
Gene ID : 22339
mRNA Refseq : NM_001025250
Protein Refseq : NP_001020421
UniProt ID : Q00731
Chromosome Location : 17 C; 17 24.2 cM
Function : growth factor activity; heparin binding

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