Active Recombinant Human MDH2

• Cat.No.: MDH2-1093H
• Product Overview: Human MDH2 (NP_005909, 25 a.a. - 338 a.a.) partial recombinant protein expressed in Escherichia coli.

Specification

• Cat. No.: MDH2-1093H
• Description: MDH2 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
• Sequence: MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK
• Theoretical MW (kDa): 35.2
• Form: Liquid
• Preparation Method: E. coli expression system
• Purity: > 95% by SDS-PAGE
• Activity: Specific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25°C.
• Application: SDS-PAGE
• Storage Buffer: In 20 mM Tris-HCl, pH 7.5 (10% glycerol)
• Storage: Store at 4°C for 1-2 weeks. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
• Unitprot ID: P40926

Gene Information

• Gene Name: MDH2 malate dehydrogenase 2, NAD (mitochondrial) [ Homo sapiens ]
• Official Symbol: MDH2
• Synonyms: MDH2; malate dehydrogenase 2, NAD (mitochondrial); OTTHUMP00000210118; malate dehydrogenase, mitochondrial; MDH; MOR1; M-MDH; MGC:3559; EC 1.1.1.37
• Gene ID: 4191
• mRNA Refseq: NM_005918
• Protein Refseq: NP_005909
• MIM: 154100
• Chromosome Location: 7cen-q22
• Function: L-malate dehydrogenase activity; malate dehydrogenase (NADP+) activity; oxidoreductase activity; protein self-association