Active Recombinant Human IL18 protein

• Cat.No.: IL18-336H
• Product Overview: Recombinant Human IL18 was expressed in E. coli.

Specification

• Description: Interleukin 18 (IL-18), previously known as interferon-gamma (IFN-gamma)-inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon.
• Source: E. coli
• Species: Human
• Form: Liquid. In Phosphate buffer saline (pH 7.4)
• Bio-activity: Measured by its ability to induce IFN-gamma secretion using KG-1 human myeloid leukemia cells in the presence of TNF-alpha (20 ng/ml). The ED50 for this effect is < 0.85 ug/ml.
• Molecular Mass: 18.3kDa (158aa), confirmed by MALDI-TOF.
• AA Sequence: MYFGKLESKL SVIRNLNDQV LFIDQGNRPL FEDMTDSDCR DNAPRTIFII SMYKDSQPRG MAVTISVKCE KISTLSCENK IISFKEMNPPDNIKDTKSDI IFFQRSVPGH DNKMQFESSS YEGYFLACEK ERDLFKLILK KEDELGDRSI MFTVQNED
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ]
• Official Symbol: IL18
• Synonyms: IL18; interleukin 18 (interferon-gamma-inducing factor); interleukin-18; IGIF; IL 1g; IL 18; IL1F4; IL-1 gamma; iboctadekin; interleukin-1 gamma; IFN-gamma-inducing factor; IL-18; IL-1g; MGC12320
• Gene ID: 3606
• mRNA Refseq: NM_001562
• Protein Refseq: NP_001553
• MIM: 600953
• UniProt ID: Q14116
• Chromosome Location: 11q22.2-q22.3
• Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Cellular roles of Anthrax toxin, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem
• Function: cytokine activity; cytokine activity