Recombinant Human MDK, His-tagged

• Cat.No.: MDK-506H
• Product Overview: The Human Midkine is created as a recombinant protein with N terminal fusion of HisTag. The Human Midkine His-Tagged Fusion Protein, produced in E. coli, is 14.6 kDa protein containing 121 amino acid residues of the human Midkine and 10 additional amino acid residues - HisTag.

Specification

• Cat. No.: MDK-506H
• Description: MDK is a basic heparin-binding growth factor of low molecular weight, a member of the NEGF family whose founding member is pleiotrophin (NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges.
• Source: E. coli
• Species: Human
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.1 M NaCl, Ph 7.2.
• Research topic: Oncology
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: ELISA; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MKHHHHHHHM KKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D
• Storage: Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Gene Information

• Gene Name: MDK midkine (neurite growth-promoting factor 2) [ Homo sapiens ]
• Official Symbol: MDK
• Synonyms: MDK; midkine (neurite growth-promoting factor 2); MK; NEGF2; FLJ27379; midkine; ARAP; OTTHUMP00000197041; OTTHUMP00000197042; OTTHUMP00000197043; OTTHUMP00000197044; OTTHUMP00000197045; OTTHUMP00000233051; OTTHUMP00000233088; amphiregulin-associated protein; midgestation and kidney protein; neurite outgrowth-promoting protein; neurite outgrowth-promoting factor 2
• Gene ID: 4192
• mRNA Refseq: NM_001012333
• Protein Refseq: NP_001012333
• MIM: 162096
• UniProt ID: P21741
• Chromosome Location: 11p11
• Pathway: Glypican 2 network; Syndecan-4-mediated signaling events
• Function: growth factor activity; heparin binding