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| Cat. No.: | MDK-506H |
| Product Overview: | The Human Midkine is created as a recombinant protein with N terminal fusion of HisTag. The Human Midkine His-Tagged Fusion Protein, produced in E. coli, is 14.6 kDa protein containing 121 amino acid residues of the human Midkine and 10 additional amino acid residues - HisTag. |
| Description: | MDK is a basic heparin-binding growth factor of low molecular weight, a member of the NEGF family whose founding member is pleiotrophin (NEGF1, 46% homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. |
| Source: | E. coli |
| Species: | Human |
| Form: | Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.1 M NaCl, Ph 7.2. |
| Research topic: | Oncology |
| Purity: | >95% |
| Reconstitution: | Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Applications: | ELISA; Western blotting |
| Quality Control Test: | BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein |
| Sequences of amino acids: | MKHHHHHHHM KKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D |
| Storage: | Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week. |
| Gene Name: | MDK midkine (neurite growth-promoting factor 2) [ Homo sapiens ] |
| Official Symbol: | MDK |
| Synonyms: | MDK; midkine (neurite growth-promoting factor 2); MK; NEGF2; FLJ27379; midkine; ARAP; OTTHUMP00000197041; OTTHUMP00000197042; OTTHUMP00000197043; OTTHUMP00000197044; OTTHUMP00000197045; OTTHUMP00000233051; OTTHUMP00000233088; amphiregulin-associated protein; midgestation and kidney protein; neurite outgrowth-promoting protein; neurite outgrowth-promoting factor 2 |
| Gene ID: | 4192 |
| mRNA Refseq: | NM_001012333 |
| Protein Refseq: | NP_001012333 |
| MIM: | 162096 |
| UniProt ID: | P21741 |
| Chromosome Location: | 11p11 |
| Pathway: | Glypican 2 network; Syndecan-4-mediated signaling events |
| Function: | growth factor activity; heparin binding |
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