||Recombinant human MPI protein, fused to His-tag at N-terminus, was expressed in E.coli (1-362aa).
||Mannose-6-phosphate isomerase, also known as MPI, belongs to the mannose-6-phosphate isomerase type 1 family, and is expressed in all tissues, more abundantly in heart, brain and skeletal muscle. Localized to the cytoplasm, MPI utilizes zinc as a cofactor and catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 5% glycerol
||41.9 kDa (382aa)
||> 90% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MAAPRVFPLS CAVQQYAWGK MGSNSEVARL LASSDPLAQI AEDKPYAELW MGTHPRGDAK ILDNRISQKT LSQWIAENQD SLGSKVKDTF NGNLPFLFKV LSVETPLSIQ AHPNKELAEK LHLQAPQHYP DANHKPEMAI ALTPFQGLCG FRPVEEIVTF LKTAAGNNME DIFGELLLQL HQQYPGDIGC FAIYFLNLLT LKPGEAMFLE ANVPHAYLKG DCVECMACSD NTVRAGLTPK FIDVPTLCEM LSYTPSSSKD RLFLPTRSQE DPYLSIYDPP VPDFTIMKTE VPGSVTEYKV LALDSASILL MVQGTVIAST PTTQTPIPLQ RGGVLFIGAN ESVSLKLTEP KDLLIFRACC LL
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Amino sugar and nucleotide sugar metabolism; Asparagine N-linked glycosylation; Fructose and mannose metabolism; Metabolic pathways; Metabolism of proteins; Post-translational protein modification; Synthesis of GDP-mannose; Synthesis of substrates in N-glycan biosythesis