||Recombinant human RGS17 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-210aa).
||RGS17, also known as regulator of G-protein signaling 17, attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. It plays an important role in termination of signalling by mu opioid receptors and development of tolerance to opioid analgesic drugs.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT
||26.5 kDa(230aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
||> 90% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MRKRQQSQNE GTPAVSQAPG NQRPNNTCCF CWCCCCSCSC LTVRNEERGE NAGRPTHTTK MESIQVLEEC QNPTAEEVLS WSQNFDKMMK APAGRNLFRE FLRTEYSEEN LLFWLACEDL KKEQNKKVIE EKARMIYEDY ISILSPKEVS LDSRVREVIN RNLLDPNPHM YEDAQLQIYT LMHRDSFPRF LNSQIYKSFV ESTAGSSSES
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Calcium Regulation in the Cardiac Cell; G alpha (z) signalling events; GPCR downstream signaling; Myometrial Relaxation and Contraction Pathways; Signaling by GPCR