||Recombinant human DENR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-198 aa).
||DENR may be involved in the translation of target mRNAs by scanning and recognition of the initiation codon. This protein plays a role in the modulation of the translational profile of a subset of cancer-related mRNAs when recruited to the translational initiation complex by the oncogene MCTS1.
||Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol, 2mM DTT, 0.15M NaCl.
||24.3 kDa (218aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
||> 85 % by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay )
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MAADISESSG ADCKGDPRNS AKLDADYPLR VLYCGVCSLP TEYCEYMPDV AKCRQWLEKN FPNEFAKLTV ENSPKQEAGI SEGQGTAGEE EEKKKQKRGG RGQIKQKKKT VPQKVTIAKI PRAKKKYVTR VCGLATFEID LKEAQRFFAQ KFSCGASVTG EDEIIIQGDF TDDIIDVIQE KWPEVDDDSI EDLGEVKK
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.