Recombinant Human Synaptotagmin 1, His-tagged

• Cat.No.: SYT1-3551H
• Product Overview: Recombinant human SYT1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: SYT1-3551H
• Description: Synaptotagmin-1(SYT1) belongs to the synaptotagmin family contains 2 C2 domains. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. SYT1 is the master switch responsible for allowing the human brain to release neurotrans- mitters.
• Source: E. coli.
• Purity: > 85% by SDS – PAGE.
• Formulation: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl.
• Molecular Weight: 29.5kDa (256aa), confirmed by MALDI-TOF
• Concentration: 0.25mg/ml (determined by Bradford assay)
• Sequences of amino acids: MEPKEEEKLG KLQYSLDYDF QNNQLLVGII QAAELPALDM GGTSDPYVKV FLLPDKKKKF ETKVHRKTLN PVFNEQFTFK VPYSELGGKT LVMAVYDFDR FSKHDIIGEF KVPMNTVDFG HVTEEWRDLQ SAEKEEQEKL GDICFSLRYV PTAGKLTVVI LEAKNLKKMD VGGLSDPYVK IHLMQNGKRL KKKKTTIKKN TLNPYYNESF SFEVPFEQIQ KVQVVVTVLD YDKIGKNDAI GKVFVGYNLE HHHHHH
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SYT1 synaptotagmin I [ Homo sapiens ]
• Synonyms: DKFZp781D2042; P65; SVP65; Synaptotagmin I; synaptotagmin-1; SYT; SYT1; sytI
• Gene ID: 6857
• mRNA Refseq: NM_005639
• Protein Refseq: NP_005630
• MIM: 185605
• UniProt ID: P21579
• Chromosome Location: 12cen-q21
• Pathway: Acetylcholine Neurotransmitter Release Cycle; Botulinum neurotoxicity; Dopamine Neurotransmitter Release Cycle; Effects of Botulinum toxin; GABA synthesis, release, reuptake and degradation; Glutamate Neurotransmitter Release Cycle;Neurotransmitter Release Cycle; Norepinephrine Neurotransmitter Release Cycle; Serotonin Neurotransmitter Release Cycle; Synaptic Transmission; Translocation of BoNT Light chain; Transmission across Chemical Synapses
• Function: 1-phosphatidylinositol binding; calcium ion binding; calcium-dependent protein binding; calmodulin binding; identical protein binding; low-density lipoprotein particle receptor binding; phospholipid binding; protein bindingsyntaxin-1 binding; transporter activity