Recombinant Human DDX58, GST-tagged

• Cat.No.: DDX58-10H
• Product Overview: Recombinant Human DDX58 (1 a.a. - 925 a.a.) fused with GST-tag at N-terminal, was expressed in vitro wheat germ expression system.

Specification

• Cat. No.: DDX58-10H
• Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response.
• Source: wheat germ
• Molecular Mass: 133 kDa
• Sequence: MTTEQRRSLQAFQDYIRKTLDPTYI LSYMAPWFREEEVQYIQAEKNNKGP MEAATLFLKFLLELQEEGWFRGFLD ALDHAGYSG LYEAIESWDFKKIEKLEEYRLLLKR LQPEFKTRIIPTDIISDLSECLINQ ECEEILQICSTKGMMAGAEKLVECL LRSDKENWPKTL KLALEKERNKFSELWIVEKGIKDVE TEDLEDKMETSDIQIFYQEDPECQN LSENSCPPSEVSDTNLYSPFKPRNY QLELALPAM KGKNTIICAPTGCGKTFVSLLICEH HLKKFPQGQKGKVVFFANQIPVYEQ QKSVFSKYFERHGYRVTGISGATAE NVPVEQIVEN NDIIILTPQILVNNLKKGTIPSLSI FTLMIFDECHNTSKQHPYNMIMFNY LDQKLGGSSGPLPQVIGLTASVGVG DAKNTDEALDYI CKLCASLDASVIATVKHNLEELEQV VYKPQKFFRKVESRISDKFKYIIAQ LMRDTESLAKRICKDLENLSQIQNR EFGTQKYEQW IVTVQKACMVFQMPDKDEESRICKA LFLYTSHLRKYNDALIISEHARMKD ALDYLKDFFSNVRAAGFDEIEQDLT QRFEEKLQEL ESVSRDPSNENPKLEDLCFILQEEY HLNPETITILFVKTRALVDALKNWI EGNPKLSFLKPGILTGRGKTNQNTG MTLPAQKCIL DAFKASGDHNILIATSVADEGIDIA QCNLVILYEYVGNVIKMIQTRGRGR ARGSKCFLLTSNAGVIEKEQINMYK EKMMNDSILRL QTWDEAVFREKILHIQTHEKFIRDS QEKPKPVPDKENKKLLCRKCKALAC YTADVRVIEECHYTVLGDAFKECFV SRPHPKPK QFSSFEKRAKIFCARQNCSHDWGIH VKYKTFEIPVIKIESFVVEDIATGV QTLYSKWKDFHFEKIPFDPAEMSK
• Purification: Glutathione Sepharose 4 Fast Flow
• Storage buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Applications: ELISA; WB
• Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
• Note: Best use within three months from the date of receipt of this protein.
• OfficialSymbol: DDX58

Gene Information

• Gene Name: DDX58 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 [ Homo sapiens ]
• Synonyms: DDX58; RIGI; RIG-I; RLR-1; RIG-1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; probable ATP-dependent RNA helicase DDX58; RNA helicase RIG-I; DEAD box protein 58; RIG-I-like receptor 1; retinoic acid inducible gene I; retinoic acid-inducible gene 1 protein; retinoic acid-inducible gene I protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; EC 3.6.4.13; DKFZp434J1111; FLJ13599
• Gene ID: 23586
• mRNA Refseq: NM_014314
• Protein Refseq: NP_055129
• MIM: 609631
• UniProt ID: O95786
• Chromosome Location: 9p12
• Pathway: Antiviral mechanism by IFN-stimulated genes; Cytokine Signaling in Immune system; Cytosolic DNA-sensing pathway; Epstein-Barr virus infection; Herpes simplex infection
• Function: ATP binding; ATP-dependent helicase activity; double-stranded DNA binding; double-stranded RNA binding; identical protein binding; protein binding; single-stranded RNA binding; zinc ion binding