Recombinant Human Lipopolysaccharide Binding Protein, His-tagged
Cat.No. : | LBP-4927H |
Product Overview : | Recombinant human LBP containing His tag was expressed by baculovirus in CHO cells and having a molecular mass of 58.0 kDa. |
- Specification
- Gene Information
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Cat. No. : | LBP-4927H |
Description : | Natural LBP is a 58 kDa glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophils and granulocytes. This is a His-tagged protein |
Biological Activity : | Up to 0.2 µg/ ml LBP mediates binding of FITC-LPS (0.5µg/ml) to CD14+CHO transfectants (FACS). |
Molecular Weight : | 58.0 kDa |
Source : | CHO cells |
Species : | Human |
Form : | Lyophilized from a concentrated protein solution (0.25mg/ml) containing PBS, pH 7.2 |
Endotoxin Level : | < 0.03 ng/ ml |
Appearance : | Lyophilized protein |
Reconstitution : | Centrifuge the vial prior to opening. Reconstitute in 40 µl of ultra-pure distilled water. This solution can then be diluted into other aqueous buffers and stored at -20°C or -70°C for future use. |
Purity : | >90-95% by SDS-PAGE analyses |
Storage : | The lyophilized protein is best-stored desiccated below 0°C. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% BSA). Repeated freezing and thawing should be avoided. |
Pathways : | Toll-like receptor signaling pathway; Signaling in Immune system |
Gene Name : | LBP lipopolysaccharide binding protein [ Homo sapiens ] |
Official Symbol : | LBP |
Synonyms : | LBP; lipopolysaccharide binding protein; BPIFD2; MGC22233; lipopolysaccharide-binding protein; OTTHUMP00000030965; LPS-binding protein; BPI fold containing family D, member 2 |
Gene ID : | 3929 |
mRNA Refseq : | NM_004139 |
Protein Refseq : | NP_004130 |
MIM : | 151990 |
UniProt ID : | P18428 |
Chromosome Location : | 20q11.23-q12 |
Function : | Gram-negative bacterial cell surface binding; Gram-positive bacterial cell surface binding; cell surface binding; lipid binding; lipopolysaccharide binding; lipoteichoic acid binding; receptor binding |
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◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionIf LPS's chemical spatial configuration prevents close contact with TLR4, it exhibits an antagonistic effect, inhibiting the cellular response.
LPS on the LBP-LPS complex, upon close physical contact with TLR4 on cells, induces TLR4 receptor dimerization or multimerization. This activates various transcription factors, leading to the expression of inflammatory mediators and cytokines.
For cells without membrane CD14 receptors (e.g., endothelial and epithelial cells), a soluble CD14 (sCD14)-LBP-LPS ternary complex forms, activating TLR4 and inducing various biological effects.
LBP stands for Lipopolysaccharide-Binding Protein.
LBP has a high affinity for the lipid A portion in the structure of Lipopolysaccharide (LPS) and forms the LPS-LBP complex.
Liver cells, similar to macrophages, express mCD14 and TLR4. In the absence of CD14 receptors, LPS can still bind to TLR4, but with relatively weaker intensity.
The LBP-LPS complex can transfer LPS to membrane CD14 (mCD14) receptors on cells like monocytes, macrophages, and neutrophils.
Customer Reviews (3)
Write a reviewWe appreciated the product's efforts to minimize its environmental footprint through sustainable manufacturing practices.
Its adaptability to various sample types and conditions made it a valuable asset for our diverse projects.
The product's versatility allowed us to apply it to a wide range of experimental techniques and research areas.
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