"CDC25B" Related Products


Recombinant Human Cell Division Cycle 25 Homolog B (S. pombe), GST-tagged

Cat. No.: CDC25B-1578H
Product Overview: Full-length recombinant human CDC25B was expressed in Sf9 insect cells using an N-terminal GST tag.
Description: CDC25B (also known as cell division cycle 25 homolog B) is a member of the CDC25 family of phosphatases that activate the cyclin dependent kinase CDC2 by removing two phosphate groups. CDC25B is required for cell entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. Cdc25B is the first genetic model for studying the mechanisms regulating prophase arrest in vertebrates. The regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
Applications: Phosphatase Assay; Western Blot
Molecular Weight: 110 kDa
Expression System: Sf9 insect cells using baculovirus
Species: Human
Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
Specific Activity: 21 nmol/min/mg
Purity: > 70 %
Concentration: 0.1 ug/ul
Sequences: Full-length
Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Pathways: Androgen Receptor Signaling Pathway; Aurora A signaling; Cell Cycle, Mitotic; Cell cycle; Cyclin A/B1 associated events during G2/M transition; Cyclin B2 mediated events; G2/M Transition; FOXM1 transcription factor network ; MAPK signaling pathway; Mitotic G2-G2/M phases; PLK1 signaling events; Progesterone-mediated oocyte maturation; S Phase; Senescence and Autophagy; p38 signaling mediated by MAPKAP kinases
Gene Name: CDC25B cell division cycle 25 homolog B (S. pombe) [ Homo sapiens ]
Official Symbol: CDC25B
Synonyms: CDC25B; cell division cycle 25 homolog B (S. pombe); cell division cycle 25B; Dual specificity phosphatase Cdc25B; cell division cycle 25 homolog B (S. cerevisiae); M-phase inducer phosphatase 2; CDC25HU2; EC
Gene ID: 994
mRNA Refseq: NM_021873
Protein Refseq: NP_068659
MIM: 116949
UniProt ID: P30305
Chromosome Location: 20p13
Function: hydrolase activity; protein binding; protein tyrosine phosphatase activity

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